Anti-Cytochrome P450 1A2 抗体 [3B8C1] (ab56073)
![Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)](https://www.abcam.co.jp/ps/products/56/ab56073/Images/ab56073-69410-anti-cytochrome-p450-1a2-antibody-3b8c1-western-blot.jpg "Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)")
Key features and details
- Mouse monoclonal [3B8C1] to Cytochrome P450 1A2
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
-
製品名
Anti-Cytochrome P450 1A2 antibody [3B8C1]
Cytochrome P450 1A2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3B8C1] to Cytochrome P450 1A2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Rat Cytochrome P450 1A2 proteins
-
ポジティブ･コントロール
- IHC-P: Normal human liver sections.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3B8C1 -
ミエローマ
Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab56073の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
|
|
| IHC-P |
Use a concentration of 1 - 5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
|
IHC-P
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
-
機能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. -
組織特異性
Liver. -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1544 Human
- Omim: 124060 Human
- SwissProt: P05177 Human
- Unigene: 1361 Human
-
別名
- Aryl hydrocarbon hydroxylase antibody
- CP 12 antibody
- CP12 antibody
see all
画像
-
Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)All lanes : Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)
Lane 2 : recombinant CYP1B1 at 0.5pmol/lane
Lane 3 : recombinant CYP1A1 at 0.5pmol/lane
Lane 4 : recombinant CYP1A2 at 0.5pmol/lane
Predicted band size: 58 kDa
Observed band size: 58 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)](https://www.abcam.co.jp/ps/products/56/ab56073/Images/ab56073-69409-anti-cytochrome-p450-1a2-antibody-3b8c1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section using ab56073.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab56073 は 6 報の論文で使用されています。
- Saha A et al. Identification of the contact region responsible for the formation of the homomeric CYP1A2•CYP1A2 complex. Biochem J 478:2163-2178 (2021). PubMed: 34032264
- Zhang X et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). PubMed: 27959388
- Higuchi Y et al. Functional polymer-dependent 3D culture accelerates the differentiation of HepaRG cells into mature hepatocytes. Hepatol Res 46:1045-57 (2016). PubMed: 26724677
- Gustafsson F et al. A correlation between the in vitro drug toxicity of drugs to cell lines that express human P450s and their propensity to cause liver injury in humans. Toxicol Sci 137:189-211 (2014). WB ; Human . PubMed: 24085192
- Fan J et al. Up-regulation of transporters and enzymes by the vitamin D receptor ligands, 1alpha,25-dihydroxyvitamin D3 and vitamin D analogs, in the Caco-2 cell monolayer. J Pharmacol Exp Ther 330:389-402 (2009). PubMed: 19414624
- Fisher CD et al. Hepatic cytochrome P450 enzyme alterations in humans with progressive stages of nonalcoholic fatty liver disease. Drug Metab Dispos 37:2087-94 (2009). PubMed: 19651758