Anti-Cytochrome P450 17A1/CYP17A1 抗体 (ab48019)
Key features and details
- Goat polyclonal to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytochrome P450 17A1/CYP17A1 antibody
Cytochrome P450 17A1/CYP17A1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Cytochrome P450 17A1/CYP17A1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Hamster, Cat, Dog, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human ovary lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48019の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
パスウェイ
Lipid metabolism; steroid biosynthesis. -
関連疾患
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
配列類似性
Belongs to the cytochrome P450 family. -
翻訳後修飾
Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 493967 Cat
- Entrez Gene: 477807 Dog
- Entrez Gene: 101831170 Hamster
- Entrez Gene: 1586 Human
- Omim: 609300 Human
- SwissProt: Q9GMC8 Cat
- SwissProt: Q8HYN1 Chimpanzee
- SwissProt: P70687 Hamster
see all -
別名
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
画像
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Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019) at 0.3 µg/ml + 35µg Human ovary lysates in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab48019 は 3 報の論文で使用されています。
- Lecante LL et al. Acetaminophen (APAP, Paracetamol) Interferes With the First Trimester Human Fetal Ovary Development in an Ex Vivo Model. J Clin Endocrinol Metab 107:1647-1661 (2022). PubMed: 35147701
- Krzeminska P et al. Altered expression of CYP17A1 and CYP19A1 in undescended testes of dogs with unilateral cryptorchidism. Anim Genet 51:763-771 (2020). PubMed: 32657440
- Santos PRS et al. Steroidogenesis during postnatal testicular development of Galea spixii. Reproduction 154:645-652 (2017). PubMed: 28982933