AB18738

Anti-Cytochrome C 抗体

Anti-Cytochrome C antibody

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(11 Publications)

Rabbit Polyclonal Cytochrome C antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 11 publications.

別名を表示する

CYC, CYCS, Cytochrome c

3 Images

Supplier Data

Western blot - Anti-Cytochrome C antibody (AB18738)

Western Blot analysis of Left Lane : Jurkat cell lysate and Right Lane : 3T3 cell lysates labeling Cytochrome C with ab18738.

All lanes:

Western blot - Anti-Cytochrome C antibody (ab18738)

Predicted band size: 11 kDa

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Supplier Data

Western blot - Anti-Cytochrome C antibody (AB18738)

All lanes:

Western blot - Anti-Cytochrome C antibody (ab18738)

All lanes:

Rat kidney cell lysates

Predicted band size: 11 kDa

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CiteAb

Western blot - Anti-Cytochrome C antibody (AB18738)

Cytochrome C western blot using anti-Cytochrome C antibody ab18738. Publication image and figure legend from Liu, H., Zhao, J., et al., 2019, PLoS One, PubMed 31091245.

ab18738 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab18738 please see the product overview.

Rk3 induces apoptosis in Eca109 and KYSE150 cells.(A) Hoechst 33342 staining. Scale bar : 100 μm. (B) Ultrastructure of apoptotic cells was observed by TEM. Scale bar : 1 μm. (C) Apoptosis was quantified by flow cytometry. (D) Effect of different doses of Rk3 on the expression of apoptosis-related proteins after treatment for 24 h. (E) Immunohistochemistry staining of tumor tissue. *p < 0.05, **p < 0.01 compared with control.

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

applications

免疫原

Synthetic Peptide within Rat Cycs. The exact immunogen used to generate this antibody is proprietary information.

P62898

特異性

This antibody detects the 15 kDa cytochrome C.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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下記製品もご検討ください

AB76237

Anti-Cytochrome C antibody [EP1326-80-5-4]

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こちらの製品も推奨する理由

私たちは、より高品質な製品をお届けするために、製品構成を常に改善しています。今回ご紹介する代替製品は、性能の向上、使いやすさや利便性の向上、またはより早くお届けできる点などを踏まえて選定しています。

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製品の詳細

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.03% Proclin 300 Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA

出荷温度

Blue Ice

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information CYCS

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federa 33:13503-13514 PubMed31570002

2019

Serine-47 phosphorylation of cytochrome in the mammalian brain regulates cytochrome oxidase and caspase-3 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hasini A Kalpage,Asmita Vaishnav,Jenney Liu,Ashwathy Varughese,Junmei Wan,Alice A Turner,Qinqin Ji,Matthew P Zurek,Alexandr A Kapralov,Valerian E Kagan,Joseph S Brunzelle,Maurice-Andre Recanati,Lawrence I Grossman,Thomas H Sanderson,Icksoo Lee,Arthur R Salomon,Brian F P Edwards,Maik Hüttemann

PloS one 14:e0216759 PubMed31091245

2019

The ginsenoside Rk3 exerts anti-esophageal cancer activity in vitro and in vivo by mediating apoptosis and autophagy through regulation of the PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Liu,Jiaqi Zhao,Rongzhan Fu,Chenhui Zhu,Daidi Fan

Experimental and therapeutic medicine 17:2152-2160 PubMed30783480

2019

MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Lusha E,Hong Jiang,Zhibing Lu

Comparative biochemistry and physiology. Part B, B 219-220:1-9 PubMed29501789

2018

A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.

Applications

Species

Unspecified reactive species

Ana Stancic,Aleksandra Jankovic,Aleksandra Korac,Dusko Cirovic,Vesna Otasevic,Kenneth B Storey,Bato Korac

Cell 171:1125-1137.e11 PubMed29107333

2017

Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program.

Applications

Unspecified application

Species

Unspecified reactive species

Farokh Dotiwala,Sumit Sen Santara,Andres Ariel Binker-Cosen,Bo Li,Sriram Chandrasekaran,Judy Lieberman

PLoS genetics 13:e1006744 PubMed28426667

2017

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

Applications

Species

Unspecified reactive species

Penelope J Boyd,Wen-Yo Tu,Hannah K Shorrock,Ewout J N Groen,Roderick N Carter,Rachael A Powis,Sophie R Thomson,Derek Thomson,Laura C Graham,Anna A L Motyl,Thomas M Wishart,J Robin Highley,Nicholas M Morton,Thomas Becker,Catherina G Becker,Paul R Heath,Thomas H Gillingwater

Biology of reproduction 91:68 PubMed25078683

2014

The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries.

Applications

Unspecified application

Species

Mouse

Sudipta Dutta,Connie J Mark-Kappeler,Patricia B Hoyer,Melissa E Pepling

Biochimica et biophysica acta 1810:1252-61 PubMed21945600

2011

Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1α and AMPKα.

Applications

Unspecified application

Species

Unspecified reactive species

Milica Vucetic,Vesna Otasevic,Aleksandra Korac,Ana Stancic,Aleksandra Jankovic,Milica Markelic,Igor Golic,Ksenija Velickovic,Biljana Buzadzic,Bato Korac

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 28:936-43 PubMed19716047

2009

Lentiviral-mediated overexpression of Bcl-xL protects primary endothelial cells from ischemia/reperfusion injury-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Eleanor Mary Bolton,John Andrew Bradley,Andrew Michael Lindsay Lever

PloS one 3:e1550 PubMed18253497

2008

Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).

Applications

Species

Human

Joseph Lemire,Ryan J Mailloux,Vasu D Appanna

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Anti-Cytochrome C 抗体

Anti-Cytochrome C antibody

Western blot - Anti-Cytochrome C antibody (AB18738)

Western blot - Anti-Cytochrome C antibody (AB18738)

Western blot - Anti-Cytochrome C antibody (AB18738)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

下記製品もご検討ください

Anti-Cytochrome C antibody [EP1326-80-5-4]

こちらの製品も推奨する理由

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

Abcam product promise

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