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AB110325

Anti-Cytochrome C 抗体 [37BA11]

Anti-Cytochrome C antibody [37BA11]

5

(4 Reviews)

|

(160 Publications)

Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody detecting Cytochrome C in Western Blot, Flow Cytometry, ICC/IF. Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat.

- Over 130 publications

別名を表示する

CYC, CYCS, Cytochrome c

5 Images
Western blot - Anti-Cytochrome C antibody [37BA11] (AB110325)
  • WB

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Western blot - Anti-Cytochrome C antibody [37BA11] (AB110325)

All lanes:

Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 5 µg

Lane 2:

Isolated mitochondria from Bovine heart at 1 µg

Lane 3:

Isolated mitochondria from Rat heart at 10 µg

Lane 4:

Isolated mitochondria from Mouse heart at 10 µg

Predicted band size: 11 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)
  • ICC/IF

AbReview47048****

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)

ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (AB110325)
  • Flow Cyt

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Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (AB110325)

Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)

Immunocytochemistry analysis using ab110325 at 2μg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325)

ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).

関連する標識済み抗体及び組成の異なる製品 (3)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11]

  • HRP

    HRP Anti-Cytochrome C antibody [37BA11]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

37BA11

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse, Rat, Cow, Human, Caenorhabditis elegans

アプリケーション

WB, ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-2 µg/mL", "ICCIF-species-notes": "<p>Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.</p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

What is this antibody validated in?
Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.

What is the molecular weight of Cytochrome C?
Anti-Cytochrome C [37BA11] (ab110325) specifically detects a band for Cytochrome C (UniProt: P99999) at a molecular weight of 12kDa.

Trusted by the scientific community
Anti-Cytochrome C [37BA11] (ab110325) was first used in a scientific publication in 2011 and has been cited over 130 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [37BA11] also available for your convenience: ab110325, Alexa Fluor® 488 - ab154476, HRP - ab198489, Alexa Fluor® 647 - ab198583

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
精製方法
Affinity purification Protein G
精製に関する特記事項
ab110325 was produced in vitro using hybridomas grown in serum-free medium, and then purified by Protein G purification via FPLC and the pure IgG fraction is eluted.
バッファー組成
pH: 7.4 - 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
See full target information CYCS
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文献 (160)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:516-526 PubMed40681898

2025

Pathology-oriented multiplexing enables integrative disease mapping.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Kuehl,Yusuke Okabayashi,Milagros N Wong,Lukas Gernhold,Gabriele Gut,Nico Kaiser,Maria Schwerk,Stefanie K Gräfe,Frank Y Ma,Jovan Tanevski,Philipp S L Schäfer,Sam Mezher,Jacobo Sarabia Del Castillo,Thiago Goldbeck-Strieder,Olga Zolotareva,Michael Hartung,Fernando M Delgado Chaves,Lukas Klinkert,Ann-Christin Gnirck,Marc Spehr,David Fleck,Mehdi Joodaki,Victor Parra,Mina Shaigan,Martin Diebold,Marco Prinz,Jennifer Kranz,Johan M Kux,Fabian Braun,Oliver Kretz,Hui Wu,Florian Grahammer,Sven Heins,Marina Zimmermann,Fabian Haas,Dominik Kylies,Nicola Wanner,Jan Czogalla,Bernhard Dumoulin,Nikolay Zolotarev,Maja Lindenmeyer,Pall Karlson,Jens R Nyengaard,Marcial Sebode,Sören Weidemann,Thorsten Wiech,Hermann-Josef Groene,Nicola M Tomas,Catherine Meyer-Schwesinger,Christoph Kuppe,Rafael Kramann,Alexandre Karras,Patrick Bruneval,Pierre-Louis Tharaux,Diego Pastene,Benito Yard,Jennifer A Schaub,Phillip J McCown,Laura Pyle,Ye Ji Choi,Takashi Yokoo,Jan Baumbach,Pablo J Sáez,Ivan Costa,Jan-Eric Turner,Jeffrey B Hodgin,Julio Saez-Rodriguez,Tobias B Huber,Petter Bjornstad,Matthias Kretzler,Olivia Lenoir,David J Nikolic-Paterson,Lucas Pelkmans,Stefan Bonn,Victor G Puelles

Molecular neurodegeneration 20:59 PubMed40389989

2025

Mutations in NEK1 cause ciliary dysfunction as a novel pathogenic mechanism in amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Young Noh,Seong-Il Oh,Young-Eun Kim,Sun Joo Cha,Wonjae Sung,Ki-Wook Oh,Yurim Park,Ji Young Mun,Chang-Seok Ki,Minyeop Nahm,Seung Hyun Kim

The Journal of reproduction and development 71:99-109 PubMed40058797

2025

Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.

Applications

Unspecified application

Species

Unspecified reactive species

Ba Anh My LE,Lien Boi Linh Nguyen,Chi Thien Lam,Nhat-Thinh Nguyen,Ngoc Thao Vy Nguyen,Van Thuan Nguyen,Hong-Thuy Bui

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70377 PubMed39985305

2025

Dolutegravir induces endoplasmic reticulum stress at the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Huang,Qing Rui Qu,Md Tozammel Hoque,Reina Bendayan

Cell discovery 11:4 PubMed39833169

2025

Apaf-1 is an evolutionarily conserved DNA sensor that switches the cell fate between apoptosis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ruan,Xuxia Wei,Suizhi Li,Zijian Ye,Linyi Hu,Ru Zhuang,Yange Cao,Shaozhou Wang,Shengpeng Wu,Dezhi Peng,Shangwu Chen,Shaochun Yuan,Anlong Xu

Veterinary medicine and science 11:e70132 PubMed39792070

2025

Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected.

Applications

Unspecified application

Species

Unspecified reactive species

Ozhan Karatas,Mustafa Ozkaraca,Mustafa O Atasoy

PLoS pathogens 20:e1012712 PubMed39689152

2024

A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haimin Li,Liuyang Du,Juan Li,Yanming Huang,Chenhe Lu,Tingjuan Deng,Yan Yan,Yulan Jin,Wei Wu,Jinyan Gu,Jiyong Zhou

Biomedicines 12: PubMed39595152

2024

Brain Injury and Neurodegeneration: Molecular, Functional, and Translational Approach 2.0.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Ahluwalia,Pankaj Gaur,Meenakshi Ahluwalia,Kumar Vaibhav

Communications biology 7:1342 PubMed39420071

2024

Protein kinase 2 of the giant sarcomeric protein UNC-89 regulates mitochondrial morphology and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Matsunaga,Hiroshi Qadota,Nasab Ghazal,Leila Lesanpezeshki,Till Dorendorf,Jasmine C Moody,Arnaud Ahier,Courtney J Matheny,Siva A Vanapalli,Steven Zuryn,Olga Mayans,Jennifer Q Kwong,Guy M Benian

Nature 632:1110-1117 PubMed39169179

2024

Lysosomes drive the piecemeal removal of mitochondrial inner membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Akriti Prashar,Claudio Bussi,Antony Fearns,Mariana I Capurro,Xiaodong Gao,Hiromi Sesaki,Maximiliano G Gutierrez,Nicola L Jones
View all publications
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代替製品

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組合せ製品

Assay Kits

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Secondary Antibodies

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