Anti-Cytochrome b5 抗体 (ab69801)
Key features and details
- Rabbit polyclonal to Cytochrome b5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Cytochrome b5 antibody
Cytochrome b5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytochrome b5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from an internal region of Human Cytochrome b5.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
|
| ICC/IF |
1/500 - 1/1000.
|
|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
|
ICC/IF
1/500 - 1/1000. |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. -
関連疾患
Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. -
配列類似性
Belongs to the cytochrome b5 family.
Contains 1 cytochrome b5 heme-binding domain. -
細胞内局在
Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1528 Human
- Entrez Gene: 109672 Mouse
- Omim: 613218 Human
- SwissProt: P00167 Human
- SwissProt: P56395 Mouse
- Unigene: 465413 Human
- Unigene: 31018 Mouse
-
別名
- CYB 5 antibody
- CYB 5A antibody
- CYB5 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b5 antibody (ab69801)IHC image of ab69801 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69801, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Cytochrome b5 antibody (ab69801)All lanes : Anti-Cytochrome b5 antibody (ab69801) at 1/500 dilution
Lane 1 : extracts from A549 cells
Lane 2 : extracts from A549 cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (ab69801)Left: NIH/3T3 cells stained with ab69801 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (11)
ab69801 は 11 報の論文で使用されています。
- Wang W et al. Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation. Onco Targets Ther 14:1007-1020 (2021). PubMed: 33603407
- Kumondai M et al. Heterologous expression of high-activity cytochrome P450 in mammalian cells. Sci Rep 10:14193 (2020). PubMed: 32843676
- Reed L et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol 366:64-74 (2019). PubMed: 30685480
- Reed L et al. Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice. Arch Toxicol 92:1625-1638 (2018). ELISA ; Mouse . PubMed: 29368147
- Stark K et al. CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women. Arthritis Res Ther 17:56 (2015). IHC-P ; Human . PubMed: 25890314