AB228751

Anti-Cytochrome b5 抗体

Anti-Cytochrome b5 antibody

JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal Cytochrome b5 antibody. Suitable for WB, ICC/IF and reacts with Human, Dog samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5A.

別名を表示する

CYB5, CYB5A, Cytochrome b5, Microsomal cytochrome b5 type A, MCB5

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Immunofluorescence analysis of PG-4 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Cytochrome b5 protein at cytoplasm using ab228751 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Immunofluorescence analysis of MDCK cells fixed in 4% paraformaldehyde at RT for 15 min labelling Cytochrome b5 protein at cytoplasm using ab228751 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Cytochrome b5 antibody (AB228751)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cytochrome b5 antibody (ab228751) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Cytochrome b5-transfected HEK-293T cell lysate

Predicted band size: 15 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Dog, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human CYB5A. The exact immunogen used to generate this antibody is proprietary information.

P00167

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome b5 also known as CYB5A is a small hemoprotein with a molecular mass of approximately 16.7 kDa. It plays a role as electron carrier for various oxidative reactions involving oxygenases and reductases. This protein exists in two soluble forms: a microsomal form found in the endoplasmic reticulum and a mitochondrial form located in the outer mitochondrial membrane. Cytochrome b5 is expressed in many tissues including liver kidney and skin where it assists in metabolic processes.

Biological function summary

The functions of Cytochrome b5 include participation in fatty acid desaturation and elongation as well as cholesterol and steroid synthesis. It interacts with various enzyme systems including the cytochrome P450 enzyme complex acting as an electron donor in these biosynthetic pathways. Cytochrome b5 contributes to the regulation of these processes ensuring proper lipid composition in cellular membranes.

Pathways

Cytochrome b5 contributes to the electron transport chain and xenobiotic metabolism pathways. In the electron transport chain it collaborates with Cytochrome P450 to facilitate monooxygenase reactions essential for metabolizing drugs and detoxification processes. Additionally Cytochrome b5 affects fatty acid metabolism by introducing double bonds into fatty acid chains interacting with stearoyl-CoA desaturase.

Cytochrome b5 plays a role in hereditary methemoglobinemia and some cases of androgen insensitivity syndrome. Mutations affecting Cytochrome b5 can disrupt electron transfer leading to elevated levels of methemoglobin and compromised oxygen delivery. In androgen insensitivity syndrome defects in the electron transfer process with other proteins like Cytochrome P450 can affect steroid metabolism and hormonal balance.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

See full target information CYB5A

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 9:596154 PubMed33634081

2021

and Are Putative Modulators of Corneoscleral Stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Boazak,Rebecca King,Jiaxing Wang,Cassandra M Chu,Aaron M Toporek,Joseph M Sherwood,Darryl R Overby,Eldon E Geisert,C Ross Ethier

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome b5 抗体

Anti-Cytochrome b5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Western blot - Anti-Cytochrome b5 antibody (AB228751)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise