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  1. Link

    products/primary-antibodies/cytochrome-b245-light-chainp22-phox-antibody-ab75941.pdf

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Anti-Cytochrome b245 Light Chain/p22-phox 抗体 (ab75941)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (24)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)

Key features and details

  • Rabbit polyclonal to Cytochrome b245 Light Chain/p22-phox
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

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関連製品

製品の概要

  • 製品名

    Anti-Cytochrome b245 Light Chain/p22-phox antibody
    Cytochrome b245 Light Chain/p22-phox 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Cytochrome b245 Light Chain/p22-phox
  • 由来種

    Rabbit
  • 特異性

    Replenishment batches of ab75941 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee. Please note, previous lots of this product were also guaranteed in ICC/IF. We are having trouble reproducing new lots suitable for ICC/IF. Therefore this application has been removed and it no longer guaranteed.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Cytochrome b245 Light Chain/p22-phox aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86646)

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Spleen Tissue. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75941の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21 kDa).
IHC-P
Use a concentration of 1 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21 kDa).
IHC-P
Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性

    Cytochrome b245 Light Chain/p22phox is a critical component of the membrane-bound oxidase complex that generates superoxide. It complexes with NOX3 to form a functional NADPH oxidase.
  • 細胞内局在

    Cell Membrane
  • 参照データベース

    • Entrez Gene: 1535 Human
    • Entrez Gene: 13057 Mouse
    • Entrez Gene: 79129 Rat
    • Omim: 608508 Human
    • SwissProt: P13498 Human
    • SwissProt: Q61462 Mouse
    • SwissProt: Q62737 Rat
    • 別名

      • CYBA antibody
      • Cytochrome b 245 alpha polypeptide antibody
      • Cytochrome b 245 light chain antibody
      • Cytochrome b alpha polypeptide antibody
      • Cytochrome b(558) alpha chain antibody
      • Cytochrome b(558) alpha subunit antibody
      • Cytochrome b558 subunit alpha antibody
      • Flavocytochrome b 558 alpha polypeptide antibody
      • Neutrophil cytochrome b 22 kDa polypeptide antibody
      • p22 phagocyte B-cytochrome antibody
      • p22-phox antibody
      • p22phox antibody
      • Superoxide-generating NADPH oxidase light chain subunit antibody
      see all

    画像

    • Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
      Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
      All lanes : Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : Pancreas (Mouse) Tissue Lysate
      Lane 3 : Spleen (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa
      Additional bands at: 19 kDa (possible cleavage fragment), 40 kDa. We are unsure as to the identity of these extra bands.



      We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain/p22-phox, and the 19 kDa band represents the mature form.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)Geng X et al., Int J Endocrinol 2016, Fig 3., doi: 10.1155/2016/2395634 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)

      IHC image of Cytochrome b245 Light Chain/p22-phox staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab75941, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (24)

    ab75941 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75941 は 24 報の論文で使用されています。

    • Kumar S  et al. Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure. Mol Biol Rep 50:2067-2076 (2023). PubMed: 36542238
    • Dilly S  et al. Targeting M2 Macrophages with a Novel NADPH Oxidase Inhibitor. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830003
    • Toma L  et al. Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells. Biomolecules 13:N/A (2023). PubMed: 37189375
    • Tang Z  et al. Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats. Aging (Albany NY) 15:12264-12274 (2023). PubMed: 37934569
    • Prado NJ  et al. Anti-inflammatory, antioxidant, antihypertensive, and antiarrhythmic effect of indole-3-carbinol, a phytochemical derived from cruciferous vegetables. Heliyon 8:e08989 (2022). PubMed: 35243102
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome b245 Light Chain/p22-phox antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 14 2017

    Western blot abreview for Anti-Cytochrome b245 Light Chain/p22-phox antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 02 2016

    Western blot abreview for Anti-Cytochrome b245 Light Chain/p22-phox antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 02 2016

    Western blot abreview for Anti-Cytochrome b245 Light Chain/p22-phox antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    50 µg
    Specification
    skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. G K Sakellariou

    Verified customer

    投稿 Sep 28 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome b245 Light Chain/p22-phox antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Single isolated FDB skeletal muscle fibres)
    Permeabilization
    Yes - 0.1% Triton X-100 (for 10 hours)
    Specification
    Single isolated FDB skeletal muscle fibres
    Blocking step
    BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. G K Sakellariou

    Verified customer

    投稿 Sep 25 2012

    Question

    Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 09 2011

    Answer

    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/NOXA2p67phox-antibody-EPR5064-ab109366.html). Click here (or use the following: https://www.abcam.com/NOX2gp91phox-antibody-ab80508.html). Click here (or use the following: https://www.abcam.com/Rac1-antibody-23A8-ab33186.html). Click here (or use the following: https://www.abcam.com/RAC2-antibody-ab2244.html). Click here (or use the following: https://www.abcam.com/RhoGDI-antibody-OTI1F2-ab118159.html). Click here (or use the following: https://www.abcam.com/Cytochrome-b245-Light-Chainp22-phox-antibody-ab75941.html). Click here (or use the following: https://www.abcam.com/p40-phox-antibody-ab115855.html). Click here (or use the following: https://www.abcam.com/p40-phox-phospho-T154-antibody-ab59355.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-phospho-S328-antibody-ab111855.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-antibody-ab795.html). Click here (or use the following: https://www.abcam.com/Human-NCF4-full-length-protein-ab88031.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NCF1p47-phox-protein-ab98054.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOXA2p67phox-protein-ab117065.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOX2gp91phox-protein-ab114183.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab84657.html). Click here (or use the following: https://www.abcam.com/Human-Rac1-full-length-protein-ab40471.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab89246.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab82670.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab51014.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab84655.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab78684.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab87665.html). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/NADPNADPH-Assay-Kit-ab65349.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Dec 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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