Anti-Cytochrome b245 Light Chain/p22-phox 抗体 [44.1] (ab80896)
![Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1] (ab80896)](https://www.abcam.co.jp/ps/products/80/ab80896/Images/ab80896-172670-anti-cytochrome-b245-light-chain-p22-phox-antibody-441-western-blot.jpg "Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1] (ab80896)")
Key features and details
- Mouse monoclonal [44.1] to Cytochrome b245 Light Chain/p22-phox
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2
製品の概要
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製品名
Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1]
Cytochrome b245 Light Chain/p22-phox 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [44.1] to Cytochrome b245 Light Chain/p22-phox -
由来種
Mouse -
特異性
This antibody is specific for Human Cytochrome b245 Light Chain/p22-phox 29-TAGRF-33 & 183-PQVNPI-188. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human Cytochrome b245 Light Chain/p22-phox. Partially Purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification – in Triton X-100)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
44.1 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2 -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80896の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent dilution. Predicted molecular weight: 21 kDa. |
ターゲット情報
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関連性
Cytochrome b245 Light Chain/p22phox is a critical component of the membrane-bound oxidase complex that generates superoxide. It complexes with NOX3 to form a functional NADPH oxidase. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 1535 Human
- Omim: 608508 Human
- SwissProt: P13498 Human
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別名
- CYBA antibody
- Cytochrome b 245 alpha polypeptide antibody
- Cytochrome b 245 light chain antibody
see all
画像
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Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1] (ab80896)
Western blot analysis of neutrophil Cytochrome b245 Light Chain/p22phox, 44.1 expression in Human spleen using ab80896
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab80896 は 9 報の論文で使用されています。
- Joshi MS et al. The EGFR phosphatase RPTPγ is a redox-regulated suppressor of promigratory signaling. EMBO J 42:e111806 (2023). PubMed: 36988334
- Gong J et al. Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats. Cell Death Discov 8:22 (2022). PubMed: 35022384
- Dakik H et al. Characterization of NADPH Oxidase Expression and Activity in Acute Myeloid Leukemia Cell Lines: A Correlation with the Differentiation Status. Antioxidants (Basel) 10:N/A (2021). PubMed: 33807114
- Hu Y et al. Downregulation of microRNA-106a-5p alleviates ox-LDL-mediated endothelial cell injury by targeting STAT3. Mol Med Rep 22:783-791 (2020). PubMed: 32626987
- Wang L et al. Methylophiopogonanone B of Radix Ophiopogonis protects cells from H2O2-induced apoptosis through the NADPH oxidase pathway in HUVECs. Mol Med Rep 20:3691-3700 (2019). PubMed: 31485606
- Ju HQ et al. Mutant Kras- and p16-regulated NOX4 activation overcomes metabolic checkpoints in development of pancreatic ductal adenocarcinoma. Nat Commun 8:14437 (2017). WB . PubMed: 28232723
- Taylor RM & Jesaitis AJ Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics. Methods Mol Biol 412:429-37 (2007). PubMed: 18453126
- Burritt JB et al. Antibody imprint of a membrane protein surface. Phagocyte flavocytochrome b. J Biol Chem 273:24847-52 (1998). PubMed: 9733789
- Burritt JB et al. Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries. J Biol Chem 270:16974-80 (1995). PubMed: 7622517