Anti-Cytochrome b-c1 complex subunit 9/UQCR10 抗体 [EPR8178] (ab181154)
![Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)](https://www.abcam.co.jp/ps/products/181/ab181154/Images/ab181154-217779-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8178-western-blot.jpg "Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8178] to Cytochrome b-c1 complex subunit 9/UQCR10
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178]
Cytochrome b-c1 complex subunit 9/UQCR10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8178] to Cytochrome b-c1 complex subunit 9/UQCR10 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HepG2, Jurkat, Saos-2 and HeLa cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8178 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181154の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/20 - 1/40.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa).
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| 特記事項 |
|---|
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IP
1/20 - 1/40. |
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WB
1/1000 - 1/10000. Detects a band of approximately 7 kDa (predicted molecular weight: 7 kDa). |
ターゲット情報
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関連性
Function: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit interacts with cytochrome c1. Similarity: Belongs to the UQCR10/QCR9 family. -
細胞内局在
Mitochondrial; Mitochondrion inner membrane -
参照データベース
- Entrez Gene: 29796 Human
- Entrez Gene: 66152 Mouse
- Omim: 610843 Human
- SwissProt: Q9UDW1 Human
- SwissProt: Q8R1I1 Mouse
- Unigene: 284292 Human
- Unigene: 269736 Mouse
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別名
- Cytochrome c1 non heme 7 kDa protein antibody
- Cytochrome C1 nonheme 7kDa protein antibody
- HSPC051 antibody
see all
画像
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Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154) at 1/5000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
![Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)](https://www.abcam.co.jp/ps/products/181/ab181154/Images/ab181154-217780-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8178-western-blot.jpg)
Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)All lanes : Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa -
![Immunoprecipitation - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)](https://www.abcam.co.jp/ps/products/181/ab181154/Images/ab181154-217781-anti-cytochrome-b-c1-complex-subunit-9-antibody-epr8178-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)Western blot analysis of Cytochrome b-c1 complex subunit 9/UQCR10 in HepG2 cell lysate immunoprecipitated with ab181154 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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![Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)](https://www.abcam.co.jp/ps/products/181/ab181154/Images/ab181154-4-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8178] (ab181154)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab181154 は論文での使用が確認できていません。