Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Cystatin S 抗体 (ab151771)

Submit a review Submit a question References (2)
Western blot - Anti-Cystatin S antibody (ab151771)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (ab151771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (ab151771)

Key features and details

  • Rabbit polyclonal to Cystatin S
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Cystatin S antibody

  • 製品の詳細

    Rabbit polyclonal to Cystatin S

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-65 of Human Cystatin S (UniProt P01036).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates, A431 cells, SW480 xenograft

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151771の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF
1/100 - 1/1000.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF
1/100 - 1/1000.

ターゲット情報

  • 機能

    This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

  • 組織特異性

    Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.

  • 配列類似性

    Belongs to the cystatin family.

  • 翻訳後修飾

    Phosphorylated at both its N- and C-terminal regions.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cst4 antibody
      • Cystatin 4 antibody
      • Cystatin S precursor antibody
      • Cystatin SA III antibody
      • Cystatin-4 antibody
      • Cystatin-S antibody
      • Cystatin-SA-III antibody
      • CYTS_HUMAN antibody
      • MGC71923 antibody
      • Salivary acidic protein 1 antibody
      see all

    画像

    • Western blot - Anti-Cystatin S antibody (ab151771)
      Western blot - Anti-Cystatin S antibody (ab151771)
      Anti-Cystatin S antibody (ab151771) at 1/1000 dilution + HepG2 whole cell
      lysate at 30 µg

      Predicted band size: 16 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (ab151771)
      Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (ab151771)
      Immunofluorescent analysis of methanol-fixed A431 cells labeling Cystatin S with ab151771 at 1/200. Lower panel co-stained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (ab151771)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (ab151771)
      Immunohistochemical analysis of paraffin embedded SW480 xenograft, labeling Cystatin S with ab151771 antibody at 1/100.

    参考文献 (2)

    ab151771 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151771 は 2 報の論文で使用されています。

    • Odanaka H  et al. Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins. Biol Res 53:3 (2020). PubMed: 31941552
    • Jain S  et al. Salivary proteome patterns of individuals exposed to High Altitude. Arch Oral Biol 96:104-112 (2018). PubMed: 30219637

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab151771.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com