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AB248523

Anti-Cystatin C 抗体 [EPR4502] - BSA and Azide free

Anti-Cystatin C antibody [EPR4502] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Cystatin C antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4502] - BSA and Azide free (AB248523)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4502] - BSA and Azide free (AB248523)

This data was developed using ab133495, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Cystatin C in paraffin embedded Human ovarian tissue, using ab133495 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Cystatin C antibody [EPR4502] - BSA and Azide free (AB248523)
  • WB

Unknown

Western blot - Anti-Cystatin C antibody [EPR4502] - BSA and Azide free (AB248523)

This data was developed using ab133495, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cystatin C antibody [EPR4502] (<a href='/products/primary-antibodies/cystatin-c-antibody-epr4502-ab133495'>ab133495</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human brain lysate at 10 µg

Lane 3:

PC3 lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 13 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4502

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

製品の詳細

ab248523 is the carrier-free version of ab133495.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Psychiatry investigation 22:699-713 PubMed40566894

2025

Transcriptional Landscape and Biomarker Discovery for Endoplasmic Reticulum Stress in Alzheimer's Disease: An Ex Vivo Study Using Patients-Derived Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Kim,You Jin Nam,Sunwoo Yoon,Young Joon Cho,Ho Min Song,Seongmin Kim,Donghyuk Shin,Jin Young Noh,Sun Min Lee,So Young Moon,Eun-Joo Kim,Soo Hyun Cho,Byeong C Kim,Seong Hye Choi,Sang Won Seo,Jin Wook Choi,Young-Sil An,Bumhee Park,Young Joon Park,Hee Young Kang,Hyun Goo Woo,Yong Hyuk Cho,Sunhwa Hong,Sang Joon Son,Sang-Rae Lee,Chang Hyung Hong,Hyun Woong Roh
View all publications

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