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AB92449

Anti-Cystatin-B 抗体 [EPR3931]

Anti-Cystatin-B antibody [EPR3931]

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Rabbit Recombinant Monoclonal Cystatin-B antibody. Suitable for IHC-P, WB and reacts with Human samples.

別名を表示する

CST6, STFB, CSTB, Cystatin-B, CPI-B, Liver thiol proteinase inhibitor, Stefin-B

4 Images
Western blot - Anti-Cystatin-B antibody [EPR3931] (AB92449)
  • WB

Lab

Western blot - Anti-Cystatin-B antibody [EPR3931] (AB92449)

Lanes 1-4 : Merged signal (red and green). Green - ab92449 observed at 15 kDa. Red - loading control ab8245 observed at 36 kDa.

ab92449 Anti-Cystatin-B antibody [EPR3931] was shown to specifically react with Cystatin-B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266321 (knockout cell lysate ab257905) was used. Wild-type and Cystatin-B knockout samples were subjected to SDS-PAGE. ab92449 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CSTB knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CSTB (Cystatin-B) knockout HEK-293T cell line (<a href='/products/cell-lines/human-cstb-cystatin-b-knockout-hek-293t-cell-line-ab266321'>ab266321</a>)

Lane 3:

U87-MG cell lysate at 20 µg

Lane 4:

JAR cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 11 kDa

Observed band size: 15 kDa

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Western blot - Anti-Cystatin-B antibody [EPR3931] (AB92449)
  • WB

Unknown

Western blot - Anti-Cystatin-B antibody [EPR3931] (AB92449)

All lanes:

Western blot - Anti-Cystatin-B antibody [EPR3931] (ab92449) at 1/1000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

Human bladder tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (AB92449)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (AB92449)

ab92449 at 1/100 dilution staining Cystatin-B in paraffin-embedded Human colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (AB92449)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin-B antibody [EPR3931] (AB92449)

ab92449 at 1/100 dilution staining Cystatin-B in paraffin-embedded Human kidney tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Cystatin-B antibody [EPR3931] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3931

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin-B also known as STEFIN-B is a protease inhibitor with a molecular mass of approximately 11 kilodaltons. It belongs to the family of cystatins which are expressed widely in human tissues with notable presence in the brain liver and kidneys. Cystatin-B acts by inhibiting cysteine proteases preventing them from degrading proteins indiscriminately. The inhibition is important for maintaining cellular protein balance and integrity.
Biological function summary

Cysteine protease inhibition by Cystatin-B protects cells from uncontrolled proteolysis which can lead to tissue damage. This protein plays an important role in the regulation of various cellular processes including apoptosis phagocytosis and the response to oxidative stress. It does not form part of a larger complex but interacts directly with its target proteases to exert its inhibitory effects.

Pathways

Cystatin-B influences proteolytic pathways by modulating the activity of cysteine proteases such as cathepsins. By inhibiting these enzymes Cystatin-B participates in the regulation of the lysosomal degradation pathway and apoptotic pathways which are important for cellular turnover and programmed cell death. Cathepsins particularly cathepsin B and L are related proteins that are significantly modulated through their interaction with Cystatin-B.

Mutations or dysregulation of Cystatin-B are associated with progressive myoclonus epilepsy and certain neurodegenerative diseases. The lack of functional Cystatin-B leads to excessive activity of proteases like cathepsin B resulting in increased proteolysis and neuronal damage. In some cases this can also connect to disorders involving oxidative stress exacerbating the detrimental cycle of cellular injury.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
See full target information CSTB
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB236646

Anti-Cystatin-B antibody

primary-antibodies

cystatin-b-antibody-ab236646

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