Anti-Cystatin A 抗体 [EPR6941] (ab128948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6941] to Cystatin A
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cystatin A antibody [EPR6941]
Cystatin A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6941] to Cystatin A -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human Cystatin A aa 50-150 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- NIH 3T3, A549, A431, K562 cell lysates; fetal brain, fetal heart, fetal kidney and Human spleen tissue lysates; NIH3T3 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR6941 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa). |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
This is an intracellular thiol proteinase inhibitor. -
配列類似性
Belongs to the cystatin family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1475 Human
- Entrez Gene: 209294 Mouse
- Entrez Gene: 288075 Rat
- Omim: 184600 Human
- SwissProt: P01040 Human
- SwissProt: P56567 Mouse
- SwissProt: P01039 Rat
- Unigene: 518198 Human
see all -
別名
- AREI antibody
- cstA antibody
- Cystatin A antibody
see all
画像
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All lanes : Anti-Cystatin A antibody [EPR6941] (ab128948) at 1/1000 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Fetal brain tissue lysate
Lane 6 : Fetal heart tissue lysate
Lane 7 : Fetal kidney tissue lysate
Lane 8 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
ab128948 at a 1/10 dilution staining Cystatin A in permeabilized NIH 3T3 cells by intracellular flow cytometry (red), with Rabbit IgG control (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab128948 は 1 報の論文で使用されています。
- Komura T et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495