Anti-CYP2U1 抗体 (ab65128)

All lanes : Anti-CYP2U1 antibody (ab65128) at 1/500 dilution

Lane 1 : HeLa cell extract
Lane 2 : LOVO cell extract
Lane 3 : LOVO cell extract with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.

Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

ICC/IF image of ab65128 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65128, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.