AB69650

Anti-CYP2S1 抗体

Name: Anti-CYP2S1 抗体 (ab69650) | Abcam
Brand: Abcam Limited
SKU: AB69650
Price: 74000 JPY
Availability: InStock

Anti-CYP2S1 antibody

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(5 Publications)

Rabbit Polyclonal CYP2S1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CYP2S1.

別名を表示する

UNQ891/PRO1906, CYP2S1, Cytochrome P450 2S1, CYPIIS1, Hydroperoxy icosatetraenoate dehydratase, Thromboxane-A synthase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2S1 antibody (AB69650)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2S1 antibody (AB69650)

ab69650 at 1/50 dilution staining CYP2S1 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-CYP2S1 antibody (AB69650)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CYP2S1 antibody (AB69650)

ab69650 at 1/500 dilution staining CYP2S1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide

Unknown

Western blot - Anti-CYP2S1 antibody (AB69650)

All lanes:

Western blot - Anti-CYP2S1 antibody (ab69650) at 1/500 dilution

Lane 1:

HUVEC cell extract at 30 µg

Lane 2:

COLO cell extract at 30 µg

Lane 3:

COLO cell extract at 30 µg with immunising peptide

Predicted band size: 56 kDa

Observed band size: 56 kDa

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CiteAb

Western blot - Anti-CYP2S1 antibody (AB69650)

CYP2S1 western blot using anti-CYP2S1 antibody ab69650. Publication image and figure legend from D'Ambrosio, D. N., Walewski, J. L., et al., 2011, PLoS One, PubMed 21949825.

ab69650 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69650 please see the product overview.

mRNA and protein expression of retinoid-metabolizing cytochrome P450 enzymes.(A) Relative mRNA expression levels of Cyp2s1 and Cyp2e1 in AF-HSCs (solid bars) and GFP-HSCs (stripped bars) are shown. Values are normalized to 18S and given as the mean ±1 S.D. for 5 AF and 5 GFP HSC isolations. Significance was determined by a Student's t-test, ** p-value<0.01. (B) Protein levels of CYP2S1 and CYP2E1 in AF-HSCs and GFP-HSCs. Experiments were repeated with similar results.

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Synthetic Peptide within Human CYP2S1. The exact immunogen used to generate this antibody is proprietary information.

Q96SQ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab69650 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CYP2S1 also known as Cytochrome P450 2S1 is a member of the cytochrome P450 superfamily of enzymes. This protein has a molecular weight of approximately 56 kDa. It is mainly expressed in extrahepatic tissues with high presence noted in the liver lungs and small intestine. Its expression is also observable in epithelial tissues and certain tumor types. The function of CYP2S1 involves oxidation reactions particularly those linked to fatty acids and xenobiotic metabolism.

Biological function summary

CYP2S1 plays an essential role in drug metabolism and the detoxification of chemicals. It participates in the processing of endogenous and exogenous compounds and operates alongside other members of the cytochrome P450 family. While CYP2S1 is not part of a conventional multi-protein complex its interactions with other proteins in the family influence its activity. Its reductase partners assist in electron transfer required for enzyme functionality.

Pathways

CYP2S1 is involved in pathways associated with xenobiotic metabolism and the degradation of specific fatty acids. It functions in these pathways by acting on substrates introduced by diet or environmental exposure. CYP1A1 and CYP2B6 are other cytochrome P450 enzymes that share interactions with CYP2S1 influencing shared metabolic and signaling pathways. This cooperation provides metabolism versatility and regulates substrate-specific detoxification.

CYP2S1 has connections to cancer and inflammatory conditions. It is especially relevant in the context of lung and breast cancer where altered expression levels are often observed. The enzymes CYP2E1 and CYP1A2 also participate in cancer-related processes due to their roles in the activation of pro-carcinogens. In inflammatory diseases the activity of CYP2S1 influences lipid mediator levels which can contribute to disease progression or suppression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids (PubMed : 12711469, PubMed : 21068195). In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 12711469). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs) (PubMed : 21068195). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage (PubMed : 21068195).

See full target information CYP2S1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112505 PubMed40454092

2025

Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via aryl hydrocarbon Receptor-HEGBC positive feedback axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yuan,Yumeng Zhang,Jianguo Xu,Feng Liu,Yingchao Liang,Beili Wang,Meihong Wu,Zhiyuan Fan,Liang Yang

The journal of pathology. Clinical research 8:550-566 PubMed35902379

2022

CYP2S1 and CYP2W1 expression is associated with patient survival in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Radhika Aiyappa-Maudsley,Sarah J Storr,Emad A Rakha,Andrew R Green,Ian O Ellis,Stewart G Martin

PloS one 6:e24993 PubMed21949825

2011

Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage.

Applications

Species

Mouse

Diana N D'Ambrosio,José L Walewski,Robin D Clugston,Paul D Berk,Richard A Rippe,William S Blaner

Molecular cancer therapeutics 10:1982-92 PubMed21831963

2011

CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells.

Applications

Species

Human

Boon Shing Tan,Kai Hung Tiong,Ashwin Muruhadas,Nirmal Randhawa,Heng Lungh Choo,Tracey D Bradshaw,Malcolm F G Stevens,Chee-Onn Leong

Molecular cancer therapeutics 10:1470-80 PubMed21622730

2011

Restitution of tumor suppressor microRNAs using a systemic nanovector inhibits pancreatic cancer growth in mice.

Applications

Species

Human

Dipankar Pramanik,Nathaniel R Campbell,Collins Karikari,Raghu Chivukula,Oliver A Kent,Joshua T Mendell,Anirban Maitra

View all publications

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Abcam product promise

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Anti-CYP2S1 抗体

Anti-CYP2S1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2S1 antibody (AB69650)

Immunocytochemistry/ Immunofluorescence - Anti-CYP2S1 antibody (AB69650)

Western blot - Anti-CYP2S1 antibody (AB69650)

Western blot - Anti-CYP2S1 antibody (AB69650)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise