Anti-CYP2J2 抗体 (ab151996)
Key features and details
- Rabbit polyclonal to CYP2J2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CYP2J2 antibody -
製品の詳細
Rabbit polyclonal to CYP2J2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CYP2J2 aa 213-472.
Database link: P51589 -
ポジティブ･コントロール
- Human breast cancer tissue; H1299, A431, HeLa, HepG2 whole cell lysate (ab7900)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools. -
組織特異性
Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney. -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1573 Human
- Omim: 601258 Human
- SwissProt: P51589 Human
- Unigene: 152096 Human
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別名
- Arachidonic acid epoxygenase antibody
- CP2J2_HUMAN antibody
- CPJ2 antibody
see all
画像
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Western blot - Anti-CYP2J2 antibody (ab151996)All lanes : Anti-CYP2J2 antibody (ab151996) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2J2 antibody (ab151996)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CYP2J2 with ab151996 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab151996 は 3 報の論文で使用されています。
- Zhang CY et al. EETs alleviate alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress through the Trim25/Keap1/Nrf2 axis. Redox Biol 63:102765 (2023). PubMed: 37269686
- Zhu X et al. Artemether inhibits proliferation, invasion and migration of hepatocellular carcinoma cells via targeting of CYP2J2. Oncol Lett 23:180 (2022). PubMed: 35464300
- Lei X et al. Targeting CYP2J2 to Enhance the Anti-Glioma Efficacy of Cannabinoid Receptor 2 Stimulation by Inhibiting the Pro-Angiogenesis Function of M2 Microglia. Front Oncol 10:574277 (2020). PubMed: 33330047