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リコンビナントRabMAb

Anti-CYP26A1 抗体 [EPR11841] (ab172474)

Reviews (1) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Western blot - Anti-CYP26A1 antibody [EPR11841] (ab172474)
  • Anti-CYP26A1 antibody [EPR11841] (ab172474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11841] to CYP26A1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CYP26A1 antibody [EPR11841]
    CYP26A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11841] to CYP26A1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat非交差種: Mouse

  • 免疫原

    Recombinant fragment corresponding to Human CYP26A1 aa 250-450.
    Database link: O43174

  • ポジティブ･コントロール

    • Human placenta, fetal liver and fetal kidney tissue lysates; 293T and HepG2 cell lysates; Human liver and pancreas tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172474の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.

  • 組織特異性

    Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.

  • 配列類似性

    Belongs to the cytochrome P450 family.

  • 細胞内局在

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CP26 antibody
      • CP26A_HUMAN antibody
      • CYP26 antibody
      • CYP26A 1 antibody
      • cyp26a1 antibody
      • Cytochrome P450 26A1 antibody
      • Cytochrome P450 family 26 subfamily A polypeptide 1 antibody
      • Cytochrome P450 retinoic acid-inactivating 1 antibody
      • cytochrome P450, family 26, subfamily A, polypeptide 1 antibody
      • cytochrome P450, subfamily XXVIA, polypeptide 1 antibody
      • Cytochrome P450RAI antibody
      • hP450RAI antibody
      • OTTHUMP00000020102 antibody
      • OTTHUMP00000020103 antibody
      • P450 antibody
      • P450 retinoic acid inactivating 1 antibody
      • P450RAI antibody
      • P450RAI1 antibody
      • Retinoic acid 4 hydroxylase antibody
      • Retinoic acid 4-hydroxylase antibody
      • Retinoic acid metabolizing cytochrome antibody
      • Retinoic acid-metabolizing cytochrome antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CYP26A1 with ab172474 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling CYP26A1 with ab172474 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      ab172474 showing +ve staining in Human normal colon tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      ab172474 showing +ve staining in Human normal uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      ab172474 showing -ve staining in Human skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP26A1 antibody [EPR11841] (ab172474)

      ab172474 showing -ve staining in Human heart muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Western blot - Anti-CYP26A1 antibody [EPR11841] (ab172474)
      All lanes : Anti-CYP26A1 antibody [EPR11841] (ab172474) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : Human fetal kidney tissue lysate
      Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    • Anti-CYP26A1 antibody [EPR11841] (ab172474)
      Anti-CYP26A1 antibody [EPR11841] (ab172474)

    参考文献 (3)

    ab172474 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab172474 は 3 報の論文で使用されています。

    • Yang N  et al. Cardiac retinoic acid levels decline in heart failure. JCI Insight 6:N/A (2021). PubMed: 33724958
    • Chen PH  et al. CYP26A1 Is a Novel Biomarker for Betel Quid-Related Oral and Pharyngeal Cancers. Diagnostics (Basel) 10:N/A (2020). PubMed: 33233443
    • Huang YJ  et al. Protection against acetaminophen-induced acute liver failure by omentum adipose tissue derived stem cells through the mediation of Nrf2 and cytochrome P450 expression. J Biomed Sci 23:5 (2016). WB ; Mouse . PubMed: 26787241

    レビューと Q&A

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    レビューを送る 質問を送る

    Western blot abreview for Anti-CYP26A1 antibody [EPR11841]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK17 cell line)
    Gel Running Conditions
    Reduced Denaturing (Gel 10%)
    Loading amount
    10 µg
    Specification
    BHK17 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Simon Boudreault

    Verified customer

    投稿 Mar 23 2017

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