Anti-Cyclophilin A 抗体 (ab41684)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cyclophilin A antibody
Cyclophilin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclophilin A -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab41684 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab126738.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Dog, Pig, Monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cyclophilin A.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41684の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
配列類似性
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Entrez Gene: 100360977 Rat
- Entrez Gene: 100362969 Rat
- Entrez Gene: 25518 Rat
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
see all -
別名
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
画像
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All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa -
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.
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ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab41684 は 54 報の論文で使用されています。
- Yilmaz DE et al. Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells. J Biol Chem 298:101589 (2022). PubMed: 35033536
- Grunwald SA et al. Statins Aggravate the Risk of Insulin Resistance in Human Muscle. Int J Mol Sci 23:N/A (2022). PubMed: 35216514
- Li L et al. miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation. Cell Death Discov 8:297 (2022). PubMed: 35760798
- Piao M et al. Cyclophilin A Promotes Osteoblast Differentiation by Regulating Runx2. Int J Mol Sci 23:N/A (2022). PubMed: 36012517
- Pang J et al. Association of Pericyte Loss With Microthrombosis After Subarachnoid Hemorrhage in ApoE-Deficient Mice. Front Neurol 12:726520 (2021). PubMed: 34566870