AB41684

Anti-Cyclophilin A 抗体

Name: Anti-Cyclophilin A 抗体 (ab41684) | Abcam
Brand: Abcam Limited
SKU: AB41684
Price: 63000 JPY
Availability: InStock
Rating: 4 (7 reviews)

Anti-Cyclophilin A antibody

(7 Reviews)

(61 Publications)

Rabbit Polyclonal Cyclophilin A antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 61 publications.

別名を表示する

CYPA, PPIA, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB41684)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB41684)

ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ICC/IF

AbReview29610****

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB41684)

Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.

This image is courtesy of an abreview submitted by Alina Macovei.

Project

Western blot - Anti-Cyclophilin A antibody (AB41684)

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab41684) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Human lymph node tissue lysate - total protein (ab29871) at 10 µg

Lane 4:

Human thymus tissue lysate - total protein (ab30146) at 10 µg

Lane 5:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Unknown

Western blot - Anti-Cyclophilin A antibody (AB41684)

ab88202 is a GST-tagged recombinant protein with an expected molecular weight of 50kDa.

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab41684) at 1 µg/mL

All lanes:

Cyclophilin A protein (Human) (ab88202) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 18 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Replenishment batches of our polyclonal antibody, ab41684 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab126738.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed : 2001362, PubMed : 20676357, PubMed : 21245143, PubMed : 21593166, PubMed : 25678563). Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (PubMed : 11943775, PubMed : 21245143). Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (PubMed : 15130913). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed : 31063815). In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (PubMed : 23180369). Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1 (PubMed : 26095851). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates (PubMed : 25678563). Plays an important role in platelet activation and aggregation (By similarity). Regulates calcium mobilization and integrin ITGA2B : ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton (By similarity). Binds heparan sulfate glycosaminoglycans (PubMed : 11943775). Inhibits replication of influenza A virus (IAV) (PubMed : 19207730). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV (PubMed : 22347431, PubMed : 30328013).. (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus (PubMed : 15688292).. (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.

See full target information PPIA

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文献 (61)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:813 PubMed40425818

2025

Deacetylation of nuclear AIF provides a braking mechanism for caspase-independent chromatinolysis and necrotic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hu,Jichuan Geng,Peipei Shan,Tongqing Zhang,Zhuqing Zhang,Xiaoyu Zhang,Menghan Lin,Xiaoxia Zhang,Dong Chang,Baokun He,Deshui Jia,Mary Zhang,Chuangui Wang,Shengping Zhang

International journal of molecular sciences 25: PubMed39684730

2024

The Impact of Resident Adipose Tissue Macrophages on Adipocyte Homeostasis and Dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Neugebauer,Nora Raulien,Lilli Arndt,Dagmar Akkermann,Constance Hobusch,Andreas Lindhorst,Janine Fröba,Martin Gericke

International journal of molecular sciences 25: PubMed39337378

2024

Replicative Endothelial Cell Senescence May Lead to Endothelial Dysfunction by Increasing the BH2/BH4 Ratio Induced by Oxidative Stress, Reducing BH4 Availability, and Decreasing the Expression of eNOS.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio Hernandez-Navarro,Laura Botana,Javier Diez-Mata,Laura Tesoro,Beatriz Jimenez-Guirado,Claudia Gonzalez-Cucharero,Nunzio Alcharani,Jose Luis Zamorano,Marta Saura,Carlos Zaragoza

Acta pharmaceutica Sinica. B 14:2520-2536 PubMed38828143

2024

Interferon-induced MXB protein restricts vimentin-dependent viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dongrong Yi,Ni An,Quanjie Li,Qian Liu,Huihan Shao,Rui Zhou,Jing Wang,Yongxin Zhang,Ling Ma,Fei Guo,Xiaoyu Li,Zhenlong Liu,Shan Cen

Cardiovascular diabetology 23:129 PubMed38622592

2024

Circ_005077 accelerates myocardial lipotoxicity induced by high-fat diet via CyPA/p47PHOX mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhu Ni,Lian Duan,Yandong Bao,Jinyang Li,Xiaowen Zhang,Dalin Jia,Nan Wu

Nature cell biology 26:593-603 PubMed38553595

2024

Cyclophilin A supports translation of intrinsically disordered proteins and affects haematopoietic stem cell ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Maneix,Polina Iakova,Charles G Lee,Shannon E Moree,Xuan Lu,Gandhar K Datar,Cedric T Hill,Eric Spooner,Jordon C K King,David B Sykes,Borja Saez,Bruno Di Stefano,Xi Chen,Daniela S Krause,Ergun Sahin,Francis T F Tsai,Margaret A Goodell,Bradford C Berk,David T Scadden,André Catic

International journal of molecular sciences 23: PubMed36012517

2022

Cyclophilin A Promotes Osteoblast Differentiation by Regulating Runx2.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyu Piao,Sung Ho Lee,Myeong Ji Kim,Joong-Kook Choi,Chang-Yeol Yeo,Kwang Youl Lee

Cell death discovery 8:297 PubMed35760798

2022

miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Shijun Yu,Jingde Chen,Ming Quan,Yong Gao,Yandong Li

International journal of molecular sciences 23: PubMed35216514

2022

Statins Aggravate the Risk of Insulin Resistance in Human Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie A Grunwald,Stefanie Haafke,Ulrike Grieben,Ursula Kassner,Elisabeth Steinhagen-Thiessen,Simone Spuler

The Journal of biological chemistry 298:101589 PubMed35033536

2022

Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Duygu Elif Yilmaz,Karin Kirschner,Hasan Demirci,Nina Himmerkus,Sebastian Bachmann,Kerim Mutig

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclophilin A 抗体

Anti-Cyclophilin A antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB41684)

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB41684)

Western blot - Anti-Cyclophilin A antibody (AB41684)

Western blot - Anti-Cyclophilin A antibody (AB41684)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (61)

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Abcam product promise