Anti-Cyclophilin A 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB3563)

Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB3563)

Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/250 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red Rhodamine Phalloidin, and the nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue). Negative control (e) is where no primary antibody was used.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (AB3563)

Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized HeLa cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).

• WB

Unknown

Western blot - Anti-Cyclophilin A antibody (AB3563)

ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab3563)

Predicted band size: 18 kDa

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• WB

Supplier Data

Western blot - Anti-Cyclophilin A antibody (AB3563)

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

K562 cell lysate at 25 µg

Lane 3:

HepG2 cell lysate at 25 µg

Lane 4:

NIH-3T3 at 25 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

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• WB

Supplier Data

Western blot - Anti-Cyclophilin A antibody (AB3563)

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab3563) at 1/3000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell extract at 30 µg

Lane 2:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, at 1/4000 dilution

Predicted band size: 18 kDa

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• WB

Supplier Data

Western blot - Anti-Cyclophilin A antibody (AB3563)

All lanes:

Western blot - Anti-Cyclophilin A antibody (ab3563) at 1/100 dilution

Lane 1:

NIH/3T3 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

mouse lung tissue lysate at 30 µg

Lane 4:

mouse colon tissue lysate at 30 µg

Lane 5:

mouse stomach tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 1/2500 dilution

Predicted band size: 18 kDa

true

• WB

CiteAb

Western blot - Anti-Cyclophilin A antibody (AB3563)

Cyclophilin A western blot using anti-Cyclophilin A antibody ab3563. Publication image and figure legend from Fan, Y. N., Meley, D., et al., 2014, PLoS One, PubMed 25250818.

ab3563 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3563 please see the product overview.

p53 activation is impaired in MEB-Med8A cells.(A) D283-MED cells were treated with [20 µM] etoposide for indicated times and the p53 protein levels were measured by western blot. (B) MEB-Med8A cells were treated with [20 µM] etoposide for indicated times and p53 protein levels were measured by western blot. 2 gels from independent experiments were quantified by densitometry analysis (AQM Advance 6 imaging software, Kinetic Imaging Ltd). The plot shown is the result of the quantification relative to cyclophilin A levels and normalised to t0 untreated control ± sd for each cell line.

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