Name: Anti-Cyclin T1 antibody [EPR17982]
Brand: RabMAb
SKU: ab184703
Rating: 3 (1 reviews)

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Western blot - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17982] to Cyclin T1
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 647 Carrier Free

製品名

Anti-Cyclin T1 antibody [EPR17982]
Cyclin T1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR17982] to Cyclin T1
由来種

Rabbit
特異性

Note that the antibody detects the target protein from human cell lysates but not tissue lysates.
アプリケーション

適用あり: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: K562, Jurkat, HeLa, HepG2, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain and spleen lysates. IHC-P: Mouse liver and rat kidney tissues. ICC/IF: K562, NIH/3T3 and PC-12 cells. Flow Cyt (intra): NIH/3T3 cells. IP: PC-12 whole cell lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR17982
アイソタイプ

IgG
研究分野

Alternative Versions
- Alexa Fluor® 647 Anti-Cyclin T1 antibody [EPR17982] (ab225325)
- Anti-Cyclin T1 antibody [EPR17982] - BSA and Azide free (ab238940)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Cyclin T1 protein (ab152261)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab184703の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/120.
WB	(1)	1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF		1/500.
IP		1/40.
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The IHC application is recommended for mouse and rat only.

特記事項
Flow Cyt (Intra) 1/120.
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/500.
IP 1/40.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The IHC application is recommended for mouse and rat only.

機能

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
組織特異性

Ubiquitously expressed.
配列類似性

Belongs to the cyclin family. Cyclin C subfamily.
細胞内局在

Nucleus.
Target information above from: UniProt accession O60563 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 904 Human
- Entrez Gene: 12455 Mouse
- Entrez Gene: 315291 Rat
- Omim: 143055 Human
- SwissProt: O60563 Human
- SwissProt: Q9QWV9 Mouse
- Unigene: 279906 Human
- Unigene: 29941 Mouse
- Unigene: 86538 Mouse
see all
別名
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
- Ccnt1 antibody
- CCNT1_HUMAN antibody
- CDK9 associated C type protein antibody
- Cyc T1 antibody
- Cyclin C related protein antibody
- Cyclin T antibody
- cyclin T1 antibody
- Cyclin T1b antibody
- Cyclin-T antibody
- Cyclin-T1 antibody
- CYCT 1 antibody
- CycT1 antibody
- HIVE1 antibody
- Human immunodeficiency virus 1 expression antibody
- Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
- pTEFb subunit antibody
- Subunit of positive elongation transcription factor b antibody
see all

Western blot - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

All lanes : Anti-Cyclin T1 antibody [EPR17982] (ab184703) at 1/1000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 81 kDa
Observed band size: 81 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Ab184703 staining Cyclin T1 in Jurkat (human T cell leukemia T lymphocyte). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1/100 dilution (6.4µg/ml). An Alexa Fluor^® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Ab195888, anti-alpha Tubulin [DM1A] – Microtubule Marker (Alexa Fluor^® 594) was used as counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing nuclear staining in Jurkat cell line.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Ab184703 staining Cyclin T1 in MCF7 (human breast adenocarcinoma epithelial cell). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:100 dilution (6.4 μg/ml). An Alexa Fluor^® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Ab195888, anti-alpha Tubulin [DM1A] – Microtubule Marker (Alexa Fluor^® 594) was used as counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing nuclear staining in MCF7 cell line.
Western blot - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

All lanes : Anti-Cyclin T1 antibody [EPR17982] (ab184703) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 81 kDa
Observed band size: 81 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2 and 3: 15 seconds; Lane 4: 3 minutes.
The antibody did not detect the target protein from human tissues (WB or IHC) but the IHC application is recommended for mouse and rat. In addition the failure of human tissue WB might result from shortage of proper human tissue.
Western blot - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

All lanes : Anti-Cyclin T1 antibody [EPR17982] (ab184703) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 81 kDa
Observed band size: 81 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2, 3 and 4: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cyclin T1 with ab184703 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Cyclin T1 with ab184703 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Cyclin T1 with ab184703 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR17982] -Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Cyclin T1 was immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate with ab184703 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184703 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: PC-12 whole cell lysate 10µg (Input).
Lane 2: ab184703 IP in PC-12 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR17982] - Isotpe Control (ab172730) instead of ab184703 in PC-12 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Anti-Cyclin T1 antibody [EPR17982] (ab184703)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab184703 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184703 は 4 報の論文で使用されています。

Shi Y et al. BRD4-targeting PROTAC as a unique tool to study biomolecular condensates. Cell Discov 9:47 (2023). PubMed: 37156794
Cheng SS et al. Inhibition of the CDK9-cyclin T1 protein-protein interaction as a new approach against triple-negative breast cancer. Acta Pharm Sin B 12:1390-1405 (2022). PubMed: 35530158
Mota de Sá P et al. Bromodomain and Extraterminal Inhibition by JQ1 Produces Divergent Transcriptional Regulation of Suppressors of Cytokine Signaling Genes in Adipocytes. Endocrinology 161:N/A (2020). PubMed: 31875887
Huang A et al. Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells. JCI Insight 4:N/A (2019). PubMed: 31484831

レビューと Q&A

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レビューを送る質問を送る

Application

Western blot

Sample

Human Cell lysate - whole cell (Pancreas)

Gel Running Conditions

Reduced Denaturing (4-12)

Loading amount

40 µg

Specification

Pancreas

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Sep 30 2022

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (4)

レビューと Q&A

Western blot abreview for Anti-Cyclin T1 antibody [EPR17982]