Anti-Cyclin C 抗体 (ab85927)
Key features and details
- Rabbit polyclonal to Cyclin C
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cyclin C antibody -
製品の詳細
Rabbit polyclonal to Cyclin C -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human Cyclin C aa 233-283.
Database link: P24863 -
ポジティブ･コントロール
- Panc1, 293T, Jurkat, H2009 and HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85927の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. -
組織特異性
Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain. -
配列類似性
Belongs to the cyclin family. Cyclin C subfamily.
Contains 1 cyclin N-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 421791 Chicken
- Entrez Gene: 509717 Cow
- Entrez Gene: 892 Human
- Entrez Gene: 51813 Mouse
- Entrez Gene: 100153639 Pig
- Entrez Gene: 114839 Rat
- Omim: 123838 Human
- SwissProt: P55168 Chicken
see all -
別名
- ccnc antibody
- CCNC_HUMAN antibody
- CycC antibody
see all
画像
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Western blot - Anti-Cyclin C antibody (ab85927)All lanes : Anti-Cyclin C antibody (ab85927) at 0.1 µg/ml
Lane 1 : Panc1 whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : H2009 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin C antibody (ab85927)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin C with ab85927 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-Cyclin C antibody (ab85927)Detection of Human Cyclin C in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85927 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab85927 は 7 報の論文で使用されています。
- Nagao S et al. C4orf47 contributes to the dormancy of pancreatic cancer under hypoxic conditions. J Cancer 14:306-317 (2023). PubMed: 36741255
- Haarhuis JHI et al. A Mediator-cohesin axis controls heterochromatin domain formation. Nat Commun 13:754 (2022). PubMed: 35136067
- Li M et al. Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway. Cancer Cell Int 21:19 (2021). PubMed: 33407495
- Liu L et al. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. Nat Commun 12:2693 (2021). PubMed: 33976158
- Lu L et al. Overexpression of miR-15b Promotes Resistance to Sunitinib in Renal Cell Carcinoma. J Cancer 10:3389-3396 (2019). PubMed: 31293642
- Xiaoli AM et al. Lipogenic SREBP-1a/c transcription factors activate expression of the iron regulator hepcidin, revealing cross-talk between lipid and iron metabolisms. J Biol Chem 294:12743-12753 (2019). PubMed: 31270208
- Fukuda A et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). PubMed: 23967313