Anti-Cyclin B2/CCNB2 抗体 [X121.10] (ab10839)
![Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)](https://www.abcam.co.jp/ps/products/10/ab10839/Images/ab10839-18596-anti-cyclin-b2-antibody-x12110-western-blot.jpg "Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)")
Key features and details
- Mouse monoclonal [X121.10] to Cyclin B2/CCNB2
- Suitable for: WB
- Reacts with: Xenopus laevis
- Isotype: IgG1
製品の概要
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製品名
Anti-Cyclin B2/CCNB2 antibody [X121.10]
Cyclin B2/CCNB2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [X121.10] to Cyclin B2/CCNB2 -
由来種
Mouse -
特異性
ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Xenopus laevis -
免疫原
Full length native protein (purified) corresponding to Xenopus laevis Cyclin B2/CCNB2.
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エピトープ
The epitope for X121 lies within a region spanning the amino acids 1-112. -
ポジティブ･コントロール
- Xenopus egg extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
X121.10 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10839の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Accumulates steadily during G2 and is abruptly destroyed at mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 397743 Xenopus laevis
- SwissProt: P13351 Xenopus laevis
- Unigene: 34562 Xenopus laevis
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別名
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
see all
画像
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Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)All lanes : Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/ml
Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab10839 は 6 報の論文で使用されています。
- Fan H et al. Decoding meningioma heterogeneity and neoplastic cell-macrophage interaction through single-cell transcriptome profiling across pathological grades. J Transl Med 21:751 (2023). PubMed: 37880655
- Li H et al. Integrative module analysis of HCC gene expression landscapes. Exp Ther Med 19:1779-1788 (2020). PubMed: 32104233
- Lin R et al. Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice. Biol Res 51:21 (2018). PubMed: 29980225
- Hansen DV et al. Emi2 at the crossroads: where CSF meets MPF. Cell Cycle 6:732-8 (2007). PubMed: 17361104
- Moore JD et al. Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 13:4388-400 (2002). PubMed: 12475960
- Bodart JF et al. Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-21 (1999). PubMed: 10585264