Anti-Cyclin B1 (phospho S126) 抗体 (ab3488)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cyclin B1 (phospho S126) antibody
Cyclin B1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin B1 (phospho S126) -
由来種
Rabbit -
特異性
This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Hamster, Dog, Chimpanzee
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免疫原
Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
Sequence: ILVDTASpPSPME
Database link: P14635 -
特記事項
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. -
一次抗体 備考
This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3488の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| 特記事項 |
|---|
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WB
1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
翻訳後修飾
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 461835 Chimpanzee
- Entrez Gene: 101836169 Hamster
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P37882 Hamster
- SwissProt: P14635 Human
see all -
別名
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
画像
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Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488)All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : H23 whole cell lysate
Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
Lane 4 : Glioblastoma (T98G) cell lysate
Lane 5 : B cell lymphoblast (Daudi) cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 20 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab3488 は 1 報の論文で使用されています。
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297