AB3488

Anti-Cyclin B1 (phospho S126) 抗体

Anti-Cyclin B1 (phospho S126) antibody

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(1 Publication)

Rabbit Polyclonal Cyclin B1 phospho S126 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCNB1 phospho S126 aa 100-150.

別名を表示する

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images

Unknown

Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)

All lanes:

Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

H23 whole cell lysate at 50 µg

Lane 3:

Hepatocarcinoma (Hep3b) cell lysate at 50 µg

Lane 4:

Glioblastoma (T98G) cell lysate at 50 µg

Lane 5:

B cell lymphoblast (Daudi) cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 48 kDa

true

Exposure time: 20s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human CCNB1 phospho S126 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P14635

特異性

This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1 phospho S126

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 16:575-584 PubMed28345297

2017

Residual Cdk1/2 activity after DNA damage promotes senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Müllers,Helena Silva Cascales,Kamila Burdova,Libor Macurek,Arne Lindqvist

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 (phospho S126) 抗体

Anti-Cyclin B1 (phospho S126) antibody

Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

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