AB18221

Anti-Cyclin B1 抗体 [V92.1]

Name: Anti-Cyclin B1 抗体 [V92.1] (ab18221) | Abcam
Brand: Abcam Limited
SKU: AB18221
Price: 72000 JPY
Availability: InStock

Anti-Cyclin B1 antibody [V92.1]

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(4 Publications)

Mouse Monoclonal Cyclin B1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human CCNB1.

別名を表示する

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (AB18221)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (AB18221)

ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

V92.1

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Full Length Protein corresponding to Human CCNB1. The exact immunogen used to generate this antibody is proprietary information.

P14635

特異性

V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 16:4185-4192 PubMed30250533

2018

Identification of key pathways and genes in lung carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Jin,Xingang Liu,Zhen Zhang,Yinghui Guan,Ren Xv,Jun Li

PLoS pathogens 13:e1006721 PubMed29121649

2017

Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Eiki Sekine,Nora Schmidt,David Gaboriau,Peter O'Hare

Oncogene 21:8089-104 PubMed12444545

2002

Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew P Garner,Claire R Weston,Daniel E Todd,Kathryn Balmanno,Simon J Cook

Experimental cell research 262:37-48 PubMed11120603

2000

Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.

Applications

Unspecified application

Species

Unspecified reactive species

Y Minemoto,J Gannon,M Masutani,H Nakagama,T Sasagawa,M Inoue,Y Masamune,K Yamashita

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 抗体 [V92.1]

Anti-Cyclin B1 antibody [V92.1]

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (AB18221)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise