Anti-Cyclin A2 抗体 [Y193] (ab32386)
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-259957-anti-cyclin-a2-antibody-y193-western-blot.jpg "Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y193] to Cyclin A2
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cyclin A2 antibody [Y193]
Cyclin A2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y193] to Cyclin A2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa, HepG2, HEK293, NIH/3T3 cell lysates. IHC-P: Skin and colon carcinoma. ICC/IF: MCF7 cells. IP: HeLa cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y193 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32386の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/40 - 1/50.
|
|
| WB | (2) |
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
For unpurified, use 1/1000. |
| ICC/IF | (3) |
1/100 - 1/250.
|
| 特記事項 |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/40 - 1/50. |
|
WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). For unpurified, use 1/1000. |
|
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
配列類似性
Belongs to the cyclin family. Cyclin AB subfamily. -
発生段階
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
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参照データベース
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Omim: 123835 Human
- SwissProt: P20248 Human
- SwissProt: P51943 Mouse
- Unigene: 58974 Human
- Unigene: 4189 Mouse
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別名
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
画像
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Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-259956-anti-cyclin-a2-antibody-y193-western-blot.jpg)
Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)All lanes : Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/10000 dilution (purified)
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-259951-anti-cyclin-a2-antibody-y193-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cyclin A2 antibody [Y193] (ab32386)ab32386 (purified) at 1/40 immunoprecipitating Cyclin A2 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 55 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-259945-anti-cyclin-a2-antibody-y193-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [Y193] (ab32386)Immunofluorescence staining of MCF7 cells with purified ab32386 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32386 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-259941-anti-cyclin-a2-antibody-y193-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [Y193] (ab32386)Immunohistochemical staining of paraffin embedded human colon carcinoma with purified ab32386 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-44671-anti-cyclin-a2-antibody-y193-western-blot.jpg)
Western blot - Anti-Cyclin A2 antibody [Y193] (ab32386)Anti-Cyclin A2 antibody [Y193] (ab32386) at 1/1000 dilution (unpurified) + HeLa cell lysate
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Anti-Cyclin A2 antibody [Y193] (ab32386)](https://www.abcam.co.jp/ps/products/32/ab32386/Images/ab32386-8-benefits-of-recombinant-antibodies.png)
Anti-Cyclin A2 antibody [Y193] (ab32386)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab32386 は 27 報の論文で使用されています。
- Witkiewicz AK et al. Determinants of response to CDK4/6 inhibitors in the real-world setting. NPJ Precis Oncol 7:90 (2023). PubMed: 37704753
- Zhao JJ et al. SNHG16 lncRNAs are overexpressed and may be oncogenic in human gastric cancer by regulating cell cycle progression. Neoplasma 69:49-58 (2022). PubMed: 34881626
- Tátrai P & Gergely F Centrosome function is critical during terminal erythroid differentiation. EMBO J 41:e108739 (2022). PubMed: 35678476
- Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
- Jiang H et al. Circadian clock core component Bmal1 dictates cell cycle rhythm of proliferating hepatocytes during liver regeneration. Am J Physiol Gastrointest Liver Physiol 321:G389-G399 (2021). PubMed: 34431407