Anti-Cyclin A2 抗体 [E23.1]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (AB38)

Immunohistochemical anaylsis of Cyclin A2 staining in Human breast cancer formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab38 (5 μg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Lab

Western blot - Anti-Cyclin A2 antibody [E23.1] (AB38)

All lanes:

Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/mL

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

true

Exposure time: 20min

• WB

AbReview469****

Western blot - Anti-Cyclin A2 antibody [E23.1] (AB38)

Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.

All lanes:

Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)

Predicted band size: 49 kDa

false

This image is courtesy of Julian Gannon, CRUK.

• WB

AbReview8935****

Western blot - Anti-Cyclin A2 antibody [E23.1] (AB38)

The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.

All lanes:

Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/mL

Lane 1:

U2OS (Hu) whole cell lysate at 30 µg

Lane 2:

293T (Hu) whole cell lysate at 30 µg

Lane 3:

Cos-7 (AGMk) whole cell lysate at 30 µg

Lane 4:

CHO (Hm) whole cell lysate at 30 µg

Lane 5:

PC12 (Rat) whole cell lysate at 30 µg

Lane 6:

3T3 (Ms) whole cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated goat anti-mouse at 1/5000 dilution

Predicted band size: 49 kDa

Observed band size: 58 kDa,64 kDa

false

Exposure time: 6min

This image is courtesy of an anonymous Abreview