AB109620

Anti-CYB5R3 抗体 [EPR3867]

Anti-CYB5R3 antibody [EPR3867]

RabMAb
Recombinant
詳細を見る

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(1 Publication)

Rabbit Recombinant Monoclonal CYB5R3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

DIA1, CYB5R3, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3867] (AB109620)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3867] (AB109620)

ab109620 at 1/250 dilution staining CYB5R3 in paraffin-embedded Human testis tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CYB5R3 antibody [EPR3867] (AB109620)

All lanes:

Western blot - Anti-CYB5R3 antibody [EPR3867] (ab109620) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 34 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3867

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat to 98°C, allow to cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein CYB5R3 also known as Cytochrome b5 reductase 3 has a molecular mass of approximately 34 kDa. This enzyme is located in various cellular compartments including the outer mitochondrial membrane and the endoplasmic reticulum. CYB5R3 plays a role in electron transfer by reducing cytochrome b5 using NADH or NADPH as electron donors. This reduction is important for various biosynthesis reactions including fatty acid desaturation and elongation as well as steroid biosynthesis.

Biological function summary

CYB5R3 contributes to the electron transport chain by facilitating the transfer of reducing equivalents to other systems. This enzyme efficiently elevates the electron flow when functioning within a larger enzymatic complex. CYB5R3 is necessary for maintaining redox balance in cells and supports the synthesis of important biomolecules like cholesterol and lipids due to its involvement in these processes.

Pathways

CYB5R3 integrates into the fatty acid metabolism and steroid biosynthesis pathways. It effectively partners with cytochrome b5 and other reductases to enable efficient electron flux within these pathways. CYB5R3's activity supports metabolic cooperation with other enzymes such as fatty acid desaturase promoting the production and transformation of essential fatty acids.

Mutations or malfunctions in CYB5R3 lead to conditions such as methemoglobinemia and insulin resistance. Methemoglobinemia results from altered oxygen delivery in the blood due to improper reduction of methemoglobin. In this context the reduced function of CYB5R3 affects the ability of related proteins like hemoglobin to bind oxygen efficiently. Insulin resistance involves metabolic alterations where CYB5R3 acts in coordination with proteins associated with lipid metabolism influencing energy homeostasis and insulin action.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.

See full target information CYB5R3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 3:588 PubMed33067514

2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Atsumi,Hiroto Katoh,Daisuke Komura,Itaru Hashimoto,Genta Furuya,Hirotomo Koda,Hiroki Konishi,Ryohei Suzuki,Asami Yamamoto,Satsuki Yuba,Hiroyuki Abe,Yasushi Rino,Takashi Oshima,Tetsuo Ushiku,Masashi Fukayama,Yasuyuki Seto,Shumpei Ishikawa

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYB5R3 抗体 [EPR3867]

Anti-CYB5R3 antibody [EPR3867]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3867] (AB109620)

Western blot - Anti-CYB5R3 antibody [EPR3867] (AB109620)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise