Anti-CYB561A3 抗体 (ab185304)
Key features and details
- Rabbit polyclonal to CYB561A3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CYB561A3 antibody -
製品の詳細
Rabbit polyclonal to CYB561A3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human CYB561A3 aa 200 to the C-terminus.
Database link: Q8NBI2 -
ポジティブ･コントロール
- IHC-P: Human stomach tissue; WB: Transfected HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185304の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Ferric-chelate reductase that reduces Fe(3+) to Fe(2+) before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor. -
配列類似性
Contains 1 cytochrome b561 domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Late endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 220002 Human
- Entrez Gene: 225912 Mouse
- SwissProt: Q8NBI2 Human
- SwissProt: Q6P1H1 Mouse
- Unigene: 22546 Human
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別名
- CYAC3_HUMAN antibody
- Cyb561a3 antibody
- CYBASC3 antibody
see all
画像
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Western blot - Anti-CYB561A3 antibody (ab185304)All lanes : Anti-CYB561A3 antibody (ab185304) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CYB561A3 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 27 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB561A3 antibody (ab185304)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling CYB561A3 in the membrane and cytoplasm of glandular cells with ab185304 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185304 は論文での使用が確認できていません。