Cy5 ® Anti-CD8 alpha 抗体 [53-6.7] (ab39850)
![Flow Cytometry - Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)](https://www.abcam.co.jp/ps/products/39/ab39850/Images/ab39850-272368-anti-cd8-alpha-antibody-53-67-cy5-flow-cytometry.jpg "Flow Cytometry - Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)")
Key features and details
- Cy5 ® Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
-
製品名
Cy5 ® Anti-CD8 alpha antibody [53-6.7]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Cy5 ® Rat monoclonal [53-6.7] to CD8 alpha -
由来種
Rat -
標識
Cy5 ®. Ex: 650nm, Em: 667nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to CD8 alpha. Spleen cells or thymocyte membranes
-
ポジティブ･コントロール
- Flow Cyt: Mouse splenocytes.
-
特記事項
In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS
Also contains a stabilizing agent. -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
ポリ/モノ
モノクローナル -
クローン名
53-6.7 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.2µg for 106 cells.
ab136584 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 0.2µg for 106 cells. ab136584 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
-
別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
-
Flow Cytometry - Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
Flow cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab39850 at 0.1 µg/106cells and CD4 with ab25659.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab39850 は 2 報の論文で使用されています。
- Mohamad NE et al. Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo. Nutr Metab (Lond) 16:49 (2019). PubMed: 31372176
- Zulkawi N et al. In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™. BMC Complement Altern Med 17:344 (2017). Flow Cyt ; Mouse . PubMed: 28666436