JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB67283

Cy3 ® Anti-LAMP1 抗体

Cy3 ® Anti-LAMP1 antibody - Lysosome Marker

5

(2 Reviews)

|

(14 Publications)

Rabbit Polyclonal LAMP1 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

2 Images
Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin. Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

標識

Cy3®

励起波長/蛍光波長

Ex: 552nm, Em: 565nm

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

ICC/IF

applications

免疫原

Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11279

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Woodchuck": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

製品の詳細

Molar Ratio (F/P): 3-9.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified using an antigen affinity matrix.The conjugate was purified by gel-filtration to remove unbound Cy3 fluorophore.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Also plays an important role in NK-cells cytotoxicity (PubMed : 2022921, PubMed : 23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed : 23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (PubMed : 7685349).. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed : 24970085, PubMed : 25972533, PubMed : 27605678, PubMed : 28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed : 29295909).. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
See full target information LAMP1
style
left-column

文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 21:1522-1534 PubMed39537902

2024

Alternative mRNA polyadenylation regulates macrophage hyperactivation via the autophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhu Chen,Baiwen Chen,Jingyu Li,Haixin Li,Gaoyang Wang,Xuemin Cai,Qianqian Zhang,Xiaoxu Liu,Chen Kan,Lei Wang,Zhengting Wang,Hua-Bing Li

iScience 27:110097 PubMed38883832

2024

Resolving neutrophils through genetic deletion of TRAM attenuate atherosclerosis pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Ran Lu,Daniel Irimia,Liwu Li

Journal of virology 97:e0028423 PubMed37255472

2023

Critical Role of Viral Protein Hexon in Hypervirulent Fowl Adenovirus Serotype-4-Induced Autophagy by Interaction with BAG3 and Promotion of Viral Replication in LMH Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Guangju You,Areayi Haiyilati,Hongnuan Wang,Huixuan Jiao,Yongqiang Wang,Li Gao,Hong Cao,Xiaoqi Li,Shijun J Zheng

International journal of molecular sciences 23: PubMed36293542

2022

Heparanase Increases Podocyte Survival and Autophagic Flux after Adriamycin-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hanan Abu-Tayeh Suleiman,Shereen Said,Haya Ali Saleh,Aviva Gamliel-Lazarovich,Eyas Haddad,Irina Minkov,Yaniv Zohar,Neta Ilan,Israel Vlodavsky,Zaid Abassi,Suheir Assady

Biophysical journal 121:1205-1218 PubMed35202608

2022

Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan T Rayens,Keisha J Cook,Scott A McKinley,Christine K Payne

JCI insight 6: PubMed34499622

2021

Resolving monocytes generated through TRAM deletion attenuate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Ziyue Yi,Ran Lu,Liwu Li

Journal of animal science and biotechnology 12:63 PubMed33966642

2021

Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Yue Zhao,Xueling Xu,Rui Zhang,Jinlong Zhang,Xiaosheng Zhang,Yan Li,Shoulong Deng,Zhengxing Lian

mSphere 6: PubMed33952660

2021

IL-1R1-Dependent Signals Improve Control of Cytosolic Virulent Mycobacteria .

Applications

Unspecified application

Species

Unspecified reactive species

Sanne van der Niet,Maaike van Zon,Karin de Punder,Anita Grootemaat,Sofie Rutten,Simone J C F M Moorlag,Diane Houben,Astrid M van der Sar,Wilbert Bitter,Roland Brosch,Rogelio Hernandez Pando,Maria T Pena,Peter J Peters,Eric A Reits,Katrin D Mayer-Barber,Nicole N van der Wel

Science advances 5:eaav2309 PubMed30775441

2019

Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Christina Lee,Liwu Li

FASEB journal : official publication of the Federa 31:4140-4152 PubMed28592636

2017

Interleukin 6 protects pancreatic β cells from apoptosis by stimulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Amelia K Linnemann,Joseph Blumer,Michelle R Marasco,Therese J Battiola,Heidi M Umhoefer,Jee Young Han,Dudley W Lamming,Dawn Belt Davis
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB25245

Anti-LAMP1 antibody [1D4B] - Lysosome Marker

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

  • 4
  • 19
Primary Antibodies

AB30687

Anti-LAMP1 antibody - Lysosome Marker

Lab Essentials

Immunocytochemistry - Anti-LAMP1 antibody - Lysosome Marker (AB30687)

  • 4
  • 4
Primary Antibodies

AB24170

Anti-LAMP1 antibody - Lysosome Marker

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (AB24170)

  • 4
  • 100
Primary Antibodies

AB208943

Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB208943)

  • 4
  • 2
Primary Antibodies

AB108597

Anti-LAMP1 antibody [EPR4204] - Lysosome Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597)

  • 4
  • 4
Primary Antibodies

AB225762

Anti-LAMP1 antibody [EPR21026] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [EPR21026] - BSA and Azide free (AB225762)

  • 0
  • 0
Primary Antibodies

AB25630

Anti-LAMP1 antibody [H4A3] - Lysosome Marker

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [H4A3] - Lysosome Marker (AB25630)

  • 4
  • 23

組合せ製品

Assay Kits

AB234047

Lysosome Isolation Kit

Functional Studies - Lysosome Isolation Kit (AB234047)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB2900

Anti-EEA1 antibody - Early Endosome Marker

KO Validated|Lab Essentials

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB2900)

  • 4
  • 39
Primary Antibodies

AB218624

Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [EPR21801] - Early Endosome Marker (AB218624)

  • 4
  • 2

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com