AB11309

Cy3 ® Anti-beta Tubulin 抗体 [TUB 2.1]

Name: Cy3 ® Anti-beta Tubulin 抗体 [TUB 2.1] (ab11309) | Abcam
Brand: Abcam Limited
SKU: AB11309
Price: 78000 JPY
Availability: InStock
Rating: 5 (11 reviews)

Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker

(11 Reviews)

(12 Publications)

Mouse Monoclonal TBB2 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Chicken samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Tubb5.

別名を表示する

Tubulin beta-2 chain, Beta-tubulin class-II

1 Images

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

Immunofluorescent analysis of methanol fixed, acetone permeabilized chicken fibroblast cells labelling beta-Tubulin-Cy3 using ab11309 at 1/200 dilution.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

TUB 2.1

アイソタイプ

IgG1

標識

Cy3®

励起波長/蛍光波長

Ex: 552nm, Em: 565nm

キャリアフリー

交差種

Chicken

アプリケーション

ICC/IF

applications

免疫原

Full Length Protein corresponding to Rat Tubb5. The exact immunogen used to generate this antibody is proprietary information.

P69897

エピトープ

Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chicken": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Proprietary technique

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TBB2_CHICK

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 9:105 PubMed37875488

2023

Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Dong,Liping Jin,Shihua Bao,Biaobang Chen,Yang Zeng,Yuxi Luo,Xingzhu Du,Qing Sang,Tianyu Wu,Lei Wang

Frontiers in immunology 13:978824 PubMed36268025

2022

Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna N Kelly,Laura Laloli,Philip V'kovski,Melle Holwerda,Jasmine Portmann,Volker Thiel,Ronald Dijkman

Frontiers in medicine 9:741989 PubMed35280870

2022

Altered Differentiation and Inflammation Profiles Contribute to Enhanced Innate Responses in Severe COPD Epithelium to Rhinovirus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Guo-Parke,Dermot Linden,Aurelie Mousnier,Ian C Scott,Helen Killick,Lee A Borthwick,Andrew J Fisher,Sinéad Weldon,Clifford C Taggart,Joseph C Kidney

Journal of biomechanics 129:110774 PubMed34627073

2021

Effects of cyclic tensile strain and microgravity on the distribution of actin fiber and Fat1 cadherin in murine articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Junpei Hatakeyama,Masato Nomura,Yoshio Wakimoto,Shota Inoue,Changxin Li,Daisuke Takamura,Toshihiro Akisue,Hideki Moriyama

Molecular & cellular proteomics : MCP 19:793-807 PubMed32075873

2020

The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Touzelet,Lindsay Broadbent,Stuart D Armstrong,Waleed Aljabr,Elaine Cloutman-Green,Ultan F Power,Julian A Hiscox

PLoS genetics 15:e1008261 PubMed31860668

2019

Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Eliska Taborska,Josef Pasulka,Radek Malik,Filip Horvat,Irena Jenickova,Zoe Jelić Matošević,Petr Svoboda

Frontiers in microbiology 10:811 PubMed31057517

2019

The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie J Vielle,Obdulio García-Nicolás,Blandina I Oliveira Esteves,Melanie Brügger,Artur Summerfield,Marco P Alves

The Journal of neuroscience : the official journal 39:2011-2024 PubMed30647150

2019

Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J Matamoros,Veronica J Tom,Di Wu,Yash Rao,David J Sharp,Peter W Baas

PloS one 13:e0201328 PubMed30517096

2018

Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year.

Applications

Unspecified application

Species

Unspecified reactive species

Helen E Groves,Hong Guo-Parke,Lindsay Broadbent,Michael D Shields,Ultan F Power

Molecular systems biology 14:e8238 PubMed30104419

2018

Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenz J Maier,Stefan M Kallenberger,Katharina Jechow,Marcel Waschow,Roland Eils,Christian Conrad

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cy3 ® Anti-beta Tubulin 抗体 [TUB 2.1]

Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (12)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise