Cy3 ® Anti-LAMP1 抗体 (ab67283)
Key features and details
- Cy3 ® Rabbit polyclonal to LAMP1
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Isotype: IgG
製品の概要
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製品名
Cy3 ® Anti-LAMP1 antibody
LAMP1 一次抗体 製品一覧 -
製品の詳細
Cy3 ® Rabbit polyclonal to LAMP1 -
由来種
Rabbit -
標識
Cy3 ®. Ex: 552nm, Em: 565nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Woodchuck
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免疫原
Synthetic peptide:
GRKRSHAGYQTI
conjugated to KLH, corresponding to C terminal amino acids 405-416 of Human LAMP1. -
ポジティブ･コントロール
- Human Hela cells, rat NRK cells and mouse 3T3 cells.
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特記事項
Molar Ratio (F/P): 3-9.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified using an antigen affinity matrix. The conjugate was purified by gel-filtration to remove unbound Cy3 fluorophore. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67283の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 - 2 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- Unigene: 494419 Human
- Unigene: 16716 Mouse
- Unigene: 475822 Mouse
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別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody (ab67283)
Ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin. Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
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Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody (ab67283)Ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab67283 は 10 報の論文で使用されています。
- Rayens NT et al. Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis. Biophys J 121:1205-1218 (2022). PubMed: 35202608
- van der Niet S et al. IL-1R1-Dependent Signals Improve Control of Cytosolic Virulent Mycobacteria In Vivo. mSphere 6:N/A (2021). PubMed: 33952660
- Li Y et al. Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis. J Anim Sci Biotechnol 12:63 (2021). PubMed: 33966642
- Geng S et al. Resolving monocytes generated through TRAM deletion attenuate atherosclerosis. JCI Insight 6:N/A (2021). PubMed: 34499622
- Geng S et al. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis. Sci Adv 5:eaav2309 (2019). PubMed: 30775441
- Linnemann AK et al. Interleukin 6 protects pancreatic ß cells from apoptosis by stimulation of autophagy. FASEB J 31:4140-4152 (2017). PubMed: 28592636
- Li D et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016). PubMed: 27440895
- Liu H et al. Immuno-potentiating pathway of HBsAg-HBIG immunogenic complex visualized. Hum Vaccin Immunother 12:77-84 (2016). PubMed: 26618396
- Heinonen MT et al. Tubulin- and actin-associating GIMAP4 is required for IFN-? secretion during Th cell differentiation. Immunol Cell Biol 93:158-66 (2015). IF . PubMed: 25287446
- Schornberg KL et al. Alpha5beta1-integrin controls ebolavirus entry by regulating endosomal cathepsins. Proc Natl Acad Sci U S A 106:8003-8 (2009). ICC/IF ; Hamster . PubMed: 19416892