Anti-CXCR5 抗体 (ab46218)
Key features and details
- Rabbit polyclonal to CXCR5
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CXCR5 antibody
CXCR5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCR5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human CXCR5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab46217) -
ポジティブ･コントロール
- IHC-P: Human Tonsil tissue. ICC/IF: Raji Cells. WB: ab46218 also gave a positive signal in Human Lymph Node tissue lysate (data not shown).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46218の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation. -
組織特異性
Expression in mature B-cells and Burkitt lymphoma cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 643 Human
- Omim: 601613 Human
- SwissProt: P32302 Human
- Unigene: 113916 Human
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別名
- BLR 1 antibody
- BLR1 antibody
- Burkitt lymphoma receptor 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody (ab46218)
ab46218 staining CXCR5 in Raji cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab46218 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody (ab46218)IHC image of ab46218 staining CXCR5 in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) second test for 20 mins. The section was then incubated with ab46218, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab46218 は 3 報の論文で使用されています。
- Knebel M et al. Prognostic impact of intra- and peritumoral immune cell subpopulations in head and neck squamous cell carcinomas - comprehensive analysis of the TCGA-HNSC cohort and immunohistochemical validation on 101 patients. Front Immunol 14:1172768 (2023). PubMed: 37383237
- Ladjemi MZ et al. Increased IgA Expression in Lung Lymphoid Follicles in Severe Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med 199:592-602 (2019). PubMed: 30339768
- Yang ZZ et al. PD-1 expression defines two distinct T-cell sub-populations in follicular lymphoma that differentially impact patient survival. Blood Cancer J 5:e281 (2015). IHC . PubMed: 25700246