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リコンビナントRabMAb

Anti-CXCL9 抗体 [EPR26512-118] - BSA and Azide free (ab290654)

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Immunocytochemistry/ Immunofluorescence - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Flow Cytometry (Intracellular) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Immunoprecipitation - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
  • Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26512-118] to CXCL9 - BSA and Azide free
  • Suitable for: ICC/IF, IP, Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free
    CXCL9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26512-118] to CXCL9 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    This antibody does not cross-react with CXCL10, CXCL11, or CXCL2 protein.

  • アプリケーション

    適用あり: ICC/IF, IP, Flow Cyt (Intra), IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Treated THP-1, treated KATO III whole cell lysates, HEK-293T cells transfected with a human CXCL9 expression vector containing a myc-His tag. Flow Cyt (intra): treated THP-1 IHC-P: treated THP-1, human prostate carcinoma and lung carcinoma tissue IP: treated KATO III whole cell lysate ICC/IF : KATO III cells.

  • 特記事項

    ab290654 is a carrier free version of ab290643. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26512-118

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290654の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
特記事項
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

ターゲット情報

  • 機能

    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

  • 配列類似性

    Belongs to the intercrine alpha (chemokine CxC) family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C-X-C motif chemokine 9 antibody
      • chemokine (C-X-C motif) ligand 9 antibody
      • CMK antibody
      • crg-10 antibody
      • CXCL9 antibody
      • CXCL9_HUMAN antibody
      • gamma interferon induced monokine antibody
      • Gamma-interferon-induced monokine antibody
      • HuMIG antibody
      • MIG antibody
      • monokine induced by gamma interferon antibody
      • monokine induced by interferon gamma antibody
      • Monokine induced by interferon-gamma antibody
      • SCYB9 antibody
      • Small inducible cytokine B9 antibody
      • small inducible cytokine subfamily B member 9 antibody
      • Small-inducible cytokine B9 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunocytochemistry/ Immunofluorescence - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KATO III (Human stomach spherical) cells labelling CXCL9 with primary antibody anti-CXCL9 (ab290643) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing increased cytoplasmic staining in KATO III cells treated with IFN gamma (human) (100 ng/ml) and TNF alpha (human) (100 ng/ml) for 16 hours. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      All lanes : Anti-CXCL9 antibody [EPR26512-118] (ab290643) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
      Lane 2 : THP-1 treated with 200ng/ml IFN gamma and 50 ng/ml LPS(Lipopolysaccharide) for 24 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      This data was developed using ab290643, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffers and concentration was 5% NFDM/TBST. 

      Exposure time: 7.75 seconds. 

      The expression of CXCL9 is upregulated in response to TNF gamma and LPS treatment (PMID:12946268, 20650898).

    • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      All lanes : Anti-Frizzled 6 antibody [EPR25319-149] (BSA and Azide free) (ab290743) at 1/1000 dilution

      Lane 1 : Untreated KATO III (human gastric carcinoma) whole cell lysate
      Lane 2 : KATO III treated with 100ng/ml IFN gamma and 100 ng/ml TNF alpha for 16 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      This data was developed using ab290643, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffers and concentration was 5% NFDM/TBST. 

      Exposure time: 5.5 seonds. 

      The expression of CXCL9 is upregulated in response to IFN gamma and TNF alpha treatment (PMID:12946268, 20650898). 

    • Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Western blot - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      All lanes : Anti-Frizzled 6 antibody [EPR25319-149] (BSA and Azide free) (ab290743) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) cells transfected with a human CXCL9 expression vector containing a myc-His tag whole cell lysate at 4 µg
      Lane 2 : HEK-293T cells transfected with a human CXCL10 expression vector containing a myc-His tag whole cell lysate at 20 µg
      Lane 3 : HEK-293T cells transfected with a human CXCL11 expression vector containing a myc-His tag whole cell lysate at 40 µg
      Lane 4 : HEK-293T cells transfected with a human CXCL2 expression vector containing a myc-His tag whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa


      Exposure time: 10 seconds


      This data was developed using ab290643, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffers and concentration was 5% NFDM/TBST. 

      Exposure time: 10 seonds. 

      This antibody does not cross-react with CXCL10, CXCL11, or CXCL2 protein. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labelling CXCL9 with ab290643 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma. The section was incubated with ab290643 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labelling CXCL9 with ab290643 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human prostate carcinoma. The section was incubated with ab290643 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Panel A THP-1 cell tissue labelling CXCL9 with ab290643 at 1/2000 (0.269 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) THP-1 cells treated with a combination of IFN-γ (200ng/ml, 24h) and LPS (50ng/ml, 24h); no staining on (B) untreated THP-1 cells. The section was incubated with ab290643 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CXCL9 with ab290643 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human cerebrum. The section was incubated with ab290643 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Flow Cytometry (Intracellular) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Flow Cytometry (Intracellular) - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde, 90% methanol THP-1 (Human monocytic leukemia monocyte) treated with 200ng/ml IFN gamma and 50ng/ml LPS for 24h (Right) labelling CXCL9 with ab290743 at 1/50 dilution/ Untreated control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. 

    • Immunoprecipitation - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Immunoprecipitation - Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

      This data was developed using ab290643, the same antibody clone in a different buffer formulation.

      CXCL9 was immunoprecipitated from 0.35 mg KATO III (human gastric carcinoma) treated with 100ng/ml IFN gamma and 100 ng/ml TNF alpha for 16 hours whole cell lysate 10 ug with ab290643 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab290643 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: KATO III (human gastric carcinoma) treated with 100ng/ml IFN gamma and 100 ng/ml TNF alpha for 16 hours whole cell lysate 10 ug

      Lane 2: ab290643 IP in KATO III treated with 100ng/ml IFN gamma and 100 ng/ml TNF alpha for 16 hours whole cell lysate

      Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab290643 in KATO III treated with 100ng/ml IFN gamma and 100 ng/ml TNF alpha for 16 hours whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3.25 seconds

    • Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)
      Anti-CXCL9 antibody [EPR26512-118] - BSA and Azide free (ab290654)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290654 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290654 は論文での使用が確認できていません。

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