Anti-CXCL9 抗体 (ab9720)
Key features and details
- Rabbit polyclonal to CXCL9
- Suitable for: ELISA, Sandwich ELISA, Neutralising, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CXCL9 antibody
CXCL9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCL9 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, Sandwich ELISA, Neutralising, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human CXCL9. Highly pure (>98%) recombinant hMIG (human Monokine induced by Interferon-Gamma)
Database link: Q07325 -
ポジティブ･コントロール
- WB: Recombinant human CXCL9 protein (ab9721).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9720の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
To detect hOPG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG. |
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required |
|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. To detect hOPG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required |
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4283 Human
- Omim: 601704 Human
- SwissProt: Q07325 Human
- Unigene: 77367 Human
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別名
- C-X-C motif chemokine 9 antibody
- chemokine (C-X-C motif) ligand 9 antibody
- CMK antibody
see all
画像
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Sandwich ELISA - Anti-CXCL9 antibody (ab9720)
Detection of hMIG (human Monokine induced by Interferon-Gamma) by sandwich ELISA (100 μl/well antibody solution). ab9720 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab9720 は 11 報の論文で使用されています。
- Lee AR et al. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model. Exp Mol Med 55:1713-1719 (2023). PubMed: 37524866
- Choi YW et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh) 8:2002497 (2021). PubMed: 33643790
- Zhang K et al. A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration. Cancer Sci 111:4041-4050 (2020). PubMed: 32860283
- Meng Z et al. Using ESTIMATE algorithm to establish an 8-mRNA signature prognosis prediction system and identify immunocyte infiltration-related genes in Pancreatic adenocarcinoma. Aging (Albany NY) 12:5048-5070 (2020). PubMed: 32181755
- Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
