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AB284855

Anti-CXCL5 + CXCL6 抗体 [EP13083] - BSA and Azide free

Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free

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Rabbit Recombinant Monoclonal CXCL5 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.

別名を表示する

ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5, GCP2, SCYB6, CXCL6, C-X-C motif chemokine 6, Chemokine alpha 3, Granulocyte chemotactic protein 2, Small-inducible cytokine B6, CKA-3, GCP-2

3 Images
Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)
  • IP

Lab

Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

CXCL5 + CXCL6 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell extract with ab198505 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab198505 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : A549 (Human lung carcinoma epithelial cell), whole cell lysate, 10µg

Lane 2 : A549 whole cell lysate with the ab198505 at 1/30 dilution

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198505 in A549 whole cell lysate

All lanes:

Immunoprecipitation - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/1000 dilution

All lanes:

A549 (Human lung carcinoma epithelial cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 12 kDa

false

Exposure time: 93s

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)
  • WB

Lab

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID : 23922745).

ab181602 was used a GAPDH loading control.

All lanes:

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/10000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) starved overnight, whole cell lysate at 15 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) starved overnight, then treated with 10ng/ml TNF-a, 10nM TPA and 0.1% BSA for 24 hours, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

false

Exposure time: 10s

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)
  • WB

Lab

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] - BSA and Azide free (AB284855)

This data was developed using ab198505, the same antibody clone in a different buffer formulation.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID : 23922745).

ab181602 was used a GAPDH loading control.

All lanes:

Western blot - Anti-CXCL5 + CXCL6 antibody [EP13083] (<a href='/products/primary-antibodies/cxcl5-cxcl6-antibody-ep13083-ab198505'>ab198505</a>) at 1/10000 dilution

Lane 1:

HEK-293T cells transfected with an empty expression vector containing His tag, hole cell lysate

Lane 2:

HEK-293T cells transfected with a human CXCL5 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate

Lane 3:

HEK-293T cells transfected with a human CXCL6 expression vector containing His tag, then treated with 600 ng/ml BFA for 3h, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

false

Exposure time: 10s

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP13083

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB212163

Human CXCL5 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

  • 0
  • 0
Sample Prep & Detection Kits

AB201798

DyLight® 405 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® (AB201798)

  • 0
  • 0
Sample Prep & Detection Kits

AB186922

Biotin Gold Conjugate (40nm, 10 OD)

Functional Studies - Biotin Gold Conjugate (40nm, 10 OD) (AB186922)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB206411

Anti-CXCL1/GRO alpha antibody [EPR19892]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-CXCL1/GRO alpha antibody [EPR19892] (AB206411)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

ab284855 is the carrier-free version of ab198505

The production method for this product has been changed from hybridoma to recombinant on 15th April 2024.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

C-X-C motif chemokine ligands 5 and 6 (CXCL5 and CXCL6) also known as ENA-78 and GCP-2 respectively are small proteins with a molecular mass of approximately 12 kDa. These chemokines belong to the CXC chemokine family and play key roles as signaling proteins. CXCL5 and CXCL6 are mainly expressed in neutrophils epithelial cells and to some extent in macrophages. When they bind to their receptor CXCR2 they induce chemotaxis guiding immune cells toward sites of inflammation or injury.
Biological function summary

They coordinate immune response by mediating neutrophil recruitment to inflammatory sites. CXCL5 and CXCL6 often work as a team binding CXCR2 and aiding in protective tissue responses. Their roles extend further as they partake in triggering angiogenesis the formation of new blood vessels under physiological or pathological conditions such as wound healing or cancer. These activities imply their involvement in complex cellular networks and interactions.

Pathways

The involvement of CXCL5 and CXCL6 arises prominently in the NF-kB pathway and the MAPK signaling pathway. These pathways integral to immune and inflammatory responses reflect the chemokines' interaction with proteins like IL-8 and TNF-alpha emphasizing their importance in host defense mechanisms and inflammatory signaling cascades. Their ability to engage with these pathways demonstrates an essential role in modulating the intensity and duration of inflammatory responses.

CXCL5 and CXCL6 relate to conditions such as cancer and rheumatoid arthritis. In cancer these chemokines can enhance tumor progression by increasing angiogenesis and attracting cells that promote tumor growth. Similarly in rheumatoid arthritis their overexpression correlates with joint inflammation and tissue damage. Proteins like VEGF-A connected to these chemokines further underline their association with pathological angiogenesis and inflammatory process regulation in disease states.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
See full target information CXCL5

追加のターゲット

CXCL6
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Abcam product promise

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