Anti-CXCL2 抗体 (ab9950)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: Sandwich ELISA, Neutralising, WB, ELISA
- Reacts with: Mouse
- Isotype: unknown
製品の概要
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製品名
Anti-CXCL2 antibody
CXCL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCL2 -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, Neutralising, WB, ELISAmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant full length protein corresponding to Mouse CXCL2. Highly pure (>98%) recombinant mMIP-2 (mouse Macrophage Inflammatory Protein-2)
Database link: P10889 -
ポジティブ･コントロール
- Recombinant mouse CXCL2 protein (ab9952) can be used as a positive control in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9950の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
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| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15 ng/ml), a concentration of 0.52 - 0.9 µg/ml of this antibody is required. |
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
| ELISA |
Use at an assay dependent concentration.
To detect mMIP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-2. |
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15 ng/ml), a concentration of 0.52 - 0.9 µg/ml of this antibody is required. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA
Use at an assay dependent concentration. To detect mMIP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-2. |
ターゲット情報
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機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 20310 Mouse
- SwissProt: P10889 Mouse
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別名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (6)
ab9950 は 6 報の論文で使用されています。
- Kantrong N et al. Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells. J Periodontol 93:1940-1950 (2022). PubMed: 35100435
- Chen X et al. Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury. Exp Ther Med 15:2356-2364 (2018). PubMed: 29456642
- Zhang Q et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . PubMed: 27510583
- Greer A et al. Site-Specific Neutrophil Migration and CXCL2 Expression in Periodontal Tissue. J Dent Res 95:946-52 (2016). PubMed: 27013641
- Zenobia C et al. Commensal bacteria-dependent select expression of CXCL2 contributes to periodontal tissue homeostasis. Cell Microbiol 15:1419-26 (2013). PubMed: 23433011
- Oue E et al. CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction. Biochem Biophys Res Commun 424:456-61 (2012). Neutralising . PubMed: 22771802
