Anti-CXCL2 抗体 (ab9841)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-CXCL2 antibody
CXCL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCL2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human CXCL2 aa 1-150.
Database link: P19875 -
ポジティブ・コントロール
- WB: Recombinant human CXCL2 protein (ab9842).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9841の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
Use 100 µl/well antibody solution. Detects of 0.2 - 0.4 ng/well of recombinant human CXCL2. |
特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Use 100 µl/well antibody solution. Detects of 0.2 - 0.4 ng/well of recombinant human CXCL2. |
ターゲット情報
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機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
画像
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To detect human CXCL2 by Western Blot analysis ab9841 can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human CXCL2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Human recombinant CXCL2 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant CXCL2 protein.
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To detect human CXCL2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9841 is required. This antigen affinity purified antibody, in conjunction with anti-CXCL2 (Biotin) (ab245843) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CXCL2 .
データシートおよび資料
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Datasheet download
参考文献 (1)
ab9841 は 1 報の論文で使用されています。
- Zhai C et al. MicroRNA‑27a‑3p regulates the proliferation and chemotaxis of pulmonary macrophages in non‑small cell lung carcinoma tissues through CXCL2. Oncol Lett 26:492 (2023). PubMed: 37854860