Anti-CXCL2 抗体 (ab9777)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: ELISA, Nucleotide Array, IHC-Fr, Sandwich ELISA, Neutralising, WB
- Reacts with: Rat
- Isotype: unknown
製品の概要
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製品名
Anti-CXCL2 antibody
CXCL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CXCL2 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, Nucleotide Array, IHC-Fr, Sandwich ELISA, Neutralising, WBmore details -
種交差性
交差種: Rat -
免疫原
Recombinant full length protein corresponding to Rat CXCL2 aa 1-100.
Database link: P30348 -
ポジティブ･コントロール
- WB: Recombinant rat CXCL2 protein (ab9778).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9777の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Nucleotide Array |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 23776670
|
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| Sandwich ELISA |
Use at an assay dependent concentration.
To detect Rat CXCL2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of ab9777 is required. This antigen affinity purified antibody, in conjunction with an Anti-Rat CXCL2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CXCL2. |
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of rGRO beta/MIP-2 (100 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required. |
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| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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Nucleotide Array
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 23776670 |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect Rat CXCL2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of ab9777 is required. This antigen affinity purified antibody, in conjunction with an Anti-Rat CXCL2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CXCL2. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of rGRO beta/MIP-2 (100 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 114105 Rat
- SwissProt: P30348 Rat
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別名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (7)
ab9777 は 7 報の論文で使用されています。
- Iwamoto S et al. RGMa Signal in Macrophages Induces Neutrophil-Related Astrocytopathy in NMO. Ann Neurol 91:532-547 (2022). PubMed: 35167145
- Memedovski Z et al. Classical and Alternative Activation of Rat Microglia Treated with Ultrapure Porphyromonas gingivalis Lipopolysaccharide In Vitro. Toxins (Basel) 12:N/A (2020). PubMed: 32438602
- Pan T et al. Knocking down TRPM2 expression reduces cell injury and NLRP3 inflammasome activation in PC12 cells subjected to oxygen-glucose deprivation. Neural Regen Res 15:2154-2161 (2020). PubMed: 32394974
- Nguyen PNN et al. miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming. Stem Cell Res Ther 8:214 (2017). WB . PubMed: 28962647
- Yu C et al. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun 6:8017 (2015). PubMed: 26281983
- Perino MG et al. Chemokine localization in bronchial angiogenesis. PLoS One 8:e66432 (2013). IHC-Fr ; Rat . PubMed: 23776670
- Shastry S & James LR Homocysteine-induced macrophage inflammatory protein-2 production by glomerular mesangial cells is mediated by PI3 Kinase and p38 MAPK. J Inflamm (Lond) 6:27 (2009). WB ; Rat . PubMed: 19781090
