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Anti-CXCL14 抗体 (ab46010)

Submit a review Q&A (1)References (11)
Sandwich ELISA - Anti-CXCL14 antibody (ab46010)

    Key features and details

    • Rabbit polyclonal to CXCL14
    • Suitable for: Sandwich ELISA, ICC/IF, IHC-P, WB, ELISA
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CXCL14 antibody
      CXCL14 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to CXCL14

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: Sandwich ELISA, ICC/IF, IHC-P, WB, ELISAmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Highly pure (>98%) recombinant hBRAK (human BRAK).

    • 特記事項

      Although some customers have been successful in IHC we no longer batch test in this application.

      The sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Lyophilized:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab46010の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.

    Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducing conditions.
    ELISA
    Use at an assay dependent concentration.

    Detection limit 0.2 - 0.4 ng/well.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.

    Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducing conditions.
    ELISA
    Use at an assay dependent concentration.

    Detection limit 0.2 - 0.4 ng/well.

    ターゲット情報

    • 機能

      Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.

    • 組織特異性

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.

    • 配列類似性

      Belongs to the intercrine alpha (chemokine CxC) family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 1110031L23Rik antibody
        • 1200006I23Rik antibody
        • AI414372 antibody
        • BMAC antibody
        • bolekine antibody
        • BRAK antibody
        • Breast and kidney antibody
        • C-X-C motif chemokine 14 antibody
        • C-X-C motif chemokine ligand 14 antibody
        • Chaemokine, CXC motif, ligand 14 antibody
        • Chemokine (C-X-C motif) ligand 14 antibody
        • Chemokine BRAK antibody
        • CXC chemokine in breast and kidney antibody
        • CXCL14 antibody
        • CXL14_HUMAN antibody
        • JSC antibody
        • Kec antibody
        • Kidney-expressed chemokine CXC antibody
        • KS1 antibody
        • MGC10687 antibody
        • MGC124510 antibody
        • MGC90667 antibody
        • MIP 2 gamma antibody
        • MIP-2G antibody
        • MIP2G antibody
        • MIP2gamma antibody
        • NJAC antibody
        • PRO273 antibody
        • PSEC0212 antibody
        • Scyb14 antibody
        • Small Inducible Cytokine B14 antibody
        • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
        • Small Inducible Cytokine subfamily B, member 14 antibody
        • Small-inducible cytokine B14 antibody
        • Tumor suppressing chemokine antibody
        • UNQ240 antibody
        see all

      画像

      • Sandwich ELISA - Anti-CXCL14 antibody (ab46010)
        Sandwich ELISA - Anti-CXCL14 antibody (ab46010)

      参考文献 (11)

      ab46010 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab46010 は 11 報の論文で使用されています。

      • Hawarden A  et al. A three protein signature fails to externally validate as a biomarker to predict surgical outcome in high-grade epithelial ovarian cancer. PLoS One 18:e0281798 (2023). PubMed: 36952534
      • Xu K  et al. Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis. Oncogenesis 10:66 (2021). PubMed: 34611125
      • Niu L  et al. Two coupled mutations abolished the binding of CEBPB to the promoter of CXCL14 that displayed an antiviral effect on PRRSV by activating IFN signaling. FASEB J 34:11257-11271 (2020). PubMed: 32648265
      • Sata Y  et al. High expression of CXCL14 is a biomarker of lung adenocarcinoma with micropapillary pattern. Cancer Sci 111:2588-2597 (2020). PubMed: 32403160
      • Ji X  et al. CXCL14 and NOS1 expression in specimens from patients with stage I-IIIA nonsmall cell lung cancer after curative resection. Medicine (Baltimore) 97:e0101 (2018). IHC ; Human . PubMed: 29517684
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      Apparently, my emails did not come through because the tiff files are too big. Here are the typical JEPEG pictures attached. I used MHCII ab primary as 1:100, secondary as 1:500. Everything else is the same with CXCL14. Every sample is the same pattern as the pictures shown which I think it is less likely for all of 30 tumors. Please let me know if you have any other questions. Thank you so much for your help.

      Your original inquiry to Abcam:

      I recently ordered your antibody Anti-CXCL14 antibody (ab46010) for immunohistochemistry on human paraffin bladder tumors. After a few trials, I found out that every sample stains positive with this ab. I am not sure it is because of your antibody non-specific which caused high background.

      This is the protocol I followed:

      Treated samples with 3% H2O2 for 20 minutes after de-paraffin and hydration, boiled samples in 10mM sodium citrate buffer for 20 minutes between 95-100C. Then blocked samples with 5% goat serum for one hour. Then used 1:300 primary ab with 2% goat serum for O/N, 1:750 biotin-labeled secondary ab with 3% goat serum for an hour, followed by 1:250 ExtrAvidin-peroxidase for an hour.

      BTW, I found the same problem with your anti-MHC antibody (ab55152).

      I am also wondering whether your product (ab80437) EXPOSE rabbit specific HRP/DAB detection IHC kit can solve this problem.

      Read More
      Answer

      Thank you, the images all arrived. I think you are correct, the TIFF files were probably too big.

      Have you been able to obtain good stains for other proteins using this protocol?

      I think the protocol is correct as it is. A customer did find that an enzymatic antigen retrieval gave a good MHC II stain of human tissue using ab55152 but I am not sure that will help with your samples. We do not know which enzyme he used but if you want to try an enzymatic antigen retrieval, I suggest using a trypsin solution. I can send more information about this if you like.The review can be found here:

      https://www.abcam.com/MHC-Class-II-antibody-ab55152.html&tab=abreviews&intabreviewid=25380

      Other antibodies may give better stains, assuming the samples have detectable amounts of CXCL14 and MHC class II. It is unlikely, though, that the EXPOSE kit ab80437 will improve the results.

      I will be happy to send free replacements. I recommend the mouse monoclonal ab128065 for detection of MHC class II:

      Click here (or use the following: https://www.abcam.com/MHC-Class-II-antibody-TDR311-ab128065.html).

      The image on the datasheet is of a stain that uses a different substrate than what you are using but the antibody will work with your reagents, assuming you have an anti-mouse IgG secondary antibody. If not, please reply and I will make a recommendation for a compatible secondary antibody.

      For CXCL14, I recommend the rabbit polyclonal ab36622:

      Click here (or use the following: https://www.abcam.com/CXCL14-antibody-ab36622.html).

      If you are using biotinylated secondary antibodies, you may want to consider using an endogenous biotin blocking kit. Your negative controls slides appear to have high background, possibly from endogenous biotin, and this may obscure the signal from CXCL14 and MHC II. We have a product for this, ab3387:

      Click here (or use the following: https://www.abcam.com/Endogenous-Avidin--Biotin-Blocking-System-ab3387.html).

      Please let me know if you need more information, and if you want to try the two antibodies that I recommend.

      Read More

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