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AB246518

Anti-CXCL13 抗体 [EPR23400-92]

Anti-CXCL13 antibody [EPR23400-92]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 詳細を見る

5

(1 Review)

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(14 Publications)

Anti-CXCL13 antibody [EPR23400-92] (ab246518) is a rabbit monoclonal antibody detecting CXCL13 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CXCL13 with ab246518 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphoid tissue in the human colon. The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CXCL13 with ab246518 at 1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on the human kidney (PMID : 19955043). The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CXCL13 with ab246518 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the germinal center in the human tonsil. The section was incubated with ab246518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  • HRP

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23400-92

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-CXCL13 antibody [EPR23400-92] (ab246518) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23400-92] also available for your convenience: ab246518, Carrier free - ab270408, Alexa Fluor® 647 - ab314944, Biotin - ab316303

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.
Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
See full target information CXCL13
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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101327 PubMed40092485

2025

YTHDF1 shapes immune-mediated hepatitis via regulating inflammatory cell recruitment and response.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Kailun Yu,Xiandan Zhang,Jiawen Li,Huilong Hu,Xusheng Deng,Siyu Zeng,Xiaoning Dong,Junru Zhao,Yongyou Zhang

Nature communications 16:2152 PubMed40038288

2025

Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Xia,Chenjia He,Zhinan Xue,Yuelan Wang,Yun Qin,Zhixiang Ren,Yupeng Huang,Han Luo,Hai-Ning Chen,Wei-Han Zhang,Li-Bin Huang,Yunying Shi,Yangjuan Bai,Bei Cai,Lanlan Wang,Feng Zhang,Maoxiang Qian,Wei Zhang,Yang Shu,Geng Yin,Heng Xu,Qibing Xie

Frontiers in immunology 15:1483497 PubMed39493749

2024

Tertiary lymphoid structure-related score as a predictor for survival prognosis and immunotherapy response in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wu,Haotian Cao,Siqi Ren,Jiaying Wu,Xin Liu,Qunxing Li,Qiuping Xu,Jiali Chen,Ruixin Wang,Suling Chen,Shijia Kuang,Binbin Xia,Yanyan Li,Liansheng Wang,Jintao Li,Bowen Li,Jinsong Li,Tianjun Lan

Nature communications 15:9097 PubMed39438438

2024

Single-cell sequencing reveals immune features of treatment response to neoadjuvant immunochemotherapy in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlin Yang,He Tian,Xiaowei Chen,Bozhao Li,Guangyu Bai,Qingyuan Cai,Jiachen Xu,Wei Guo,Shuaibo Wang,Yue Peng,Qing Liang,Liyan Xue,Shugeng Gao

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309755 PubMed39136172

2024

Dissecting the Distinct Tumor Microenvironments of HRD and HRP Ovarian Cancer: Implications for Targeted Therapies to Overcome PARPi Resistance in HRD Tumors and Refractoriness in HRP Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Qiu,Tingting Ren,Qinqin Liu,Qian Jiang,Tong Wu,Leong Chi Cheng,Wenqing Yan,Xinyu Qu,Xiao Han,Keqin Hua

BMC cancer 24:758 PubMed38914961

2024

Constructing a prognostic model for colon cancer: insights from immunity-related genes.

Applications

Unspecified application

Species

Unspecified reactive species

Ansu Li,Qi Li,Chaoshan Wang,Xue Bao,Feng Sun,Xiaoping Qian,Wu Sun

Scientific reports 14:13432 PubMed38862586

2024

Combined oral low-dose cyclophosphamide endocrine therapy may improve clinical response among patients with metastatic breast cancer via Tregs in TLSs.

Applications

Unspecified application

Species

Unspecified reactive species

Yuze Zhao,Shuo Wang,Shuzhen Lv,Xiaojun Liu,Weiping Li,Yuguang Song,Dongwen Rong,Peiming Zheng,Hongyan Huang,Huixia Zheng

Nature communications 15:2595 PubMed38519500

2024

Single cell deciphering of progression trajectories of the tumor ecosystem in head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Z L Liu,X Y Meng,R J Bao,M Y Shen,J J Sun,W D Chen,F Liu,Y He

JCI insight 9: PubMed38478516

2024

A distinct tumor microenvironment makes anaplastic thyroid cancer more lethal but immunotherapy sensitive than papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Zhen Han,Wei-Dong Ye,Peng-Cheng Yu,Li-Cheng Tan,Xiao Shi,Xu-Feng Chen,Cong He,Jia-Qian Hu,Wen-Jun Wei,Zhong-Wu Lu,Ning Qu,Yu Wang,Qing-Hai Ji,Dong-Mei Ji,Yu-Long Wang

Nature communications 14:610 PubMed36739462

2023

Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Lin,Xiao-Long Zhang,Rui You,You-Ping Liu,Hong-Min Cai,Li-Zhi Liu,Xue-Fei Liu,Xiong Zou,Yu-Long Xie,Ru-Hai Zou,Yi-Nuan Zhang,Rui Sun,Wei-Yi Feng,Hai-Yan Wang,Gui-Hua Tao,Hao-Jiang Li,Wen-Jie Huang,Chao Zhang,Pei-Yu Huang,Jin Wang,Qi Zhao,Qi Yang,Hong-Wan Zhang,Ting Liu,Hui-Feng Li,Xiao-Bing Jiang,Jun Tang,Yang-Kui Gu,Tao Yu,Zhi-Qiang Wang,Lin Feng,Tie-Bang Kang,Zhi-Xiang Zuo,Ming-Yuan Chen
View all publications
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