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AB322902

Anti-CXCL11 抗体 [EPR27375-62]

Anti-CXCL11 antibody [EPR27375-62]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 詳細を見る

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Rabbit Recombinant Monoclonal CXCL11 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

別名を表示する

ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells transfected with a CXCL11 expression vector containing a his tag, Panel B HEK-293T cells transfected with a CXCL9 expression vector containing a his tag, Panel C HEK-293T cells transfected with a CXCL10 expression vector containing a his tag, Panel D HEK-293T cells transfected with a CXCL2 expression vector containing a his tag and Panel E HEK-293T cells transfected with empty vector containing a his tag labeling CXCL11 with ab322902 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a CXCL11 expression vector, no staining on (B) HEK-293T transfected with a CXCL9 expression vector, (C) HEK-293T transfected with a CXCL10 expression vector, (D) HEK-293T transfected with a CXCL2 expression vector, and (E) HEK-293T transfected with empty vector.
The section was incubated with ab322902 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)

Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling CXCL11 with ab322902 at 1/100 (5.17 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human prostate cancer.
The section was incubated with ab322902 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL11 antibody [EPR27375-62] (AB322902)

Immunohistochemical analysis of paraffin-embedded Panel A untreated THP-1 cells, Panel B THP-1 cells treated with a combination of IFN-γ (200ng/ml, 24h) and LPS (50ng/ml, 24h) and Panel C THP-1 cells treated with a combination of IFN-γ (200ng/ml 16h+8h), LPS (50ng/ml 16h+8h) add BFA (300ng/ml 8h) labeling CXCL11 with ab322902 at 1/20 (25.85 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on (C) THP-1 cells treated with a combination of IFN-γ, LPS add BFA; weak staining on (B) THP-1 cells treated with a combination of IFN-γ and LPS; no staining on (A) untreated THP-1 cells.
The section was incubated with ab322902 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR27375-62

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The IHC-P application was recommended based on cell pellets. For tissues, the positive staining was only observed in one case of human prostate cancer.

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組合せ製品

Assay Kits

AB289695

Human CXCL11 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
See full target information CXCL11
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Abcam product promise

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