AB124731

Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma 抗体

Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal CXCL1/GRO alpha antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 2 publications.

別名を表示する

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

3 Images

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Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

All lanes:

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (ab124731) at 1/10000 dilution

All lanes:

CXCL1/GRO alpha recombinant protein at 0.005 µg

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Supplier Data

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (ab124731) at 1/1000 dilution

Lane 1:

His-tagged Human CXCL1(GRO alpha) recombinant protein (aa35-107) at 10 ng

Lane 2:

His-tagged Human CXCL2(GRO beta) recombinant protein (aa35-107) at 10 ng

Lane 3:

His-tagged Human CXCL3(GRO gamma) recombinant protein (aa35-107) at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 7s

OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

OI-RD Scanning

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OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4903

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody cross-reacts with recombinant human CXCL2 and CXCL3 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha CXCL2 and GRO gamma refers to chemokines that are important in immune response. These chemokines are small proteins with a mass of about 8 kDa. They signal through the activation of the chemokine receptor CXCR2. CXCL1/GRO alpha CXCL2 and GRO gamma express in various tissues including epithelial cells and immune cells like neutrophils. They function by recruiting these immune cells to sites of inflammation or injury.

Biological function summary

CXCL1/GRO alpha CXCL2 and GRO gamma are involved in the recruitment and activation of neutrophils. Neutrophils are the first responders in the immune response. These chemokines play a role in angiogenesis and wound healing by stimulating the migration and proliferation of endothelial cells. They do not function as part of a larger protein complex but operate independently in signaling pathways. Their chemotactic activities are essential for proper immune surveillance and response to infections.

Pathways

These chemokines are integral to the NF-kB signaling pathway and MAPK pathway. Through these pathways they mediate inflammatory responses and cellular proliferation. CXCL1/GRO alpha and its family members interact with proteins such as CXCR2 and MAPK which help relay signals for gene expression related to inflammation and cell survival. Their interaction with these pathways highlights their role in initiating and modulating inflammatory processes.

CXCL1/GRO alpha CXCL2 and GRO gamma have associations with inflammatory diseases like rheumatoid arthritis and cancer especially melanoma. In rheumatoid arthritis these chemokines contribute to the recruitment of neutrophils and other immune cells to the joints leading to chronic inflammation. In cancer particularly melanoma they can promote tumor growth by enhancing angiogenesis and suppressing immune response due to the involvement of CXCR2 signaling. Understanding these chemokines involves examining proteins like CXCR2 which play a direct role in disease progression through these inflammatory and cancer pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

See full target information CXCL1

追加のターゲット

CXCL2,CXCL3

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39408697

2024

Transcriptome Profiling Associated with Overexpression in Colorectal Cancer Implicates a Potential Role for Tumor Immune Microenvironment and Cancer Pathways Modulation via NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Faisal Alhosani,Burcu Yener Ilce,Reem Sami Alhamidi,Poorna Manasa Bhamidimarri,Alaa Mohamed Hamad,Noura Alkhayyal,Axel Künstner,Cyrus Khandanpour,Hauke Busch,Basel Al-Ramadi,Kadria Sayed,Ali AlFazari,Riyad Bendardaf,Rifat Hamoudi

Inflammopharmacology 30:2345-2357 PubMed36131109

2022

Melatonin ameliorates lung cell inflammation and apoptosis caused by Klebsiella pneumoniae via AMP-activated protein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Jiang,Jun Liu,Xuequn Zhao,Wenjie Yang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma 抗体

Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903]

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

Western blot - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

OI-RD Scanning - Anti-CXCL1/GRO alpha + CXCL2 + GRO gamma antibody [EPR4903] (AB124731)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (2)

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Abcam product promise