Anti-CX3CR1 抗体 (ab8020)
Key features and details
- Rabbit polyclonal to CX3CR1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CX3CR1 antibody
CX3CR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CX3CR1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.
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ポジティブ･コントロール
- THP-1 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
ターゲット情報
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機能
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
組織特異性
Expressed in lymphoid and neural tissues. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1524 Human
- Entrez Gene: 13051 Mouse
- Entrez Gene: 171056 Rat
- Omim: 601470 Human
- SwissProt: P49238 Human
- SwissProt: Q9Z0D9 Mouse
- SwissProt: P35411 Rat
- Unigene: 78913 Human
see all -
別名
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
画像
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Western blot - Anti-CX3CR1 antibody (ab8020)All lanes : Anti-CX3CR1 antibody (ab8020) at 1 µg/ml
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : CX3CR1 knockout THP-1 cell lysate
Lane 3 : Human Spleen cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CX3CR1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab8020 was shown to bind specifically to CX3CR1. A band was observed at 42 kDa in wild-type THP-1 cell lysates with no signal observed at this size in CX3CR1 knockout cell line ab273713 (knockout cell lysate ab275510). To generate this image, wild-type and CX3CR1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8020)ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC
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Western blot - Anti-CX3CR1 antibody (ab8020)All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution
Lane 1 : THP-1 cell lysate with absence of blocking peptide
Lane 2 : THP-1 cell lysate with presence of blocking peptide
Predicted band size: 50 kDa
Observed band size: 50 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab8020 は 15 報の論文で使用されています。
- Song X et al. Cochlear resident macrophage mediates development of ribbon synapses via CX3CR1/CX3CL1 axis. Front Mol Neurosci 15:1031278 (2022). PubMed: 36518186
- Chen X et al. CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage. J Neuroinflammation 17:209 (2020). PubMed: 32664984
- Chrobok NL et al. Tissue Transglutaminase Appears in Monocytes and Macrophages but Not in Lymphocytes in White Matter Multiple Sclerosis Lesions. J Neuropathol Exp Neurol 78:492-500 (2019). PubMed: 31058279
- Szukiewicz D et al. Anti-inflammatory Action of Metformin with Respect to CX3CL1/CX3CR1 Signaling in Human Placental Circulation in Normal-Glucose Versus High-Glucose Environments. Inflammation 41:2246-2264 (2018). PubMed: 30097812
- Löfroos AB et al. Colorectal cancer-infiltrating T lymphocytes display a distinct chemokine receptor expression profile. Eur J Med Res 22:40 (2017). Flow Cyt ; Human . PubMed: 29020986