Anti-Cullin 5/CUL-5 抗体 [EPR14725] (ab184177)
![Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.co.jp/ps/products/184/ab184177/Images/ab184177-333647-anti-cullin-5-cul-5-antibody-epr14725-western-blot.jpg "Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cullin 5/CUL-5 antibody [EPR14725]
Cullin 5/CUL-5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14725] to Cullin 5/CUL-5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Hela, MOLT4 or T47-D cell lysates. Human mouse and Rat heart tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14725 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184177の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 90 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 90 kDa). |
ターゲット情報
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機能
Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the cullin family. -
翻訳後修飾
Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). - Information by UniProt
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参照データベース
- Entrez Gene: 8065 Human
- Entrez Gene: 75717 Mouse
- Entrez Gene: 64624 Rat
- Omim: 601741 Human
- SwissProt: Q93034 Human
- SwissProt: Q9D5V5 Mouse
- SwissProt: Q9JJ31 Rat
- Unigene: 440320 Human
see all -
別名
- CUL 5 antibody
- CUL-5 antibody
- CUL5 antibody
see all
画像
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Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/2000 dilution (Purified)
Lane 1 : Human heart lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.co.jp/ps/products/184/ab184177/Images/ab184177-216523-anti-cullin-5-cul-5-antibody-epr14725-western-blot.jpg)
Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)All lanes : Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MOLT-4 cell lysate
Lane 3 : T47-D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)](https://www.abcam.co.jp/ps/products/184/ab184177/Images/ab184177-3-benefits-of-recombinant-antibodies.png)
Anti-Cullin 5/CUL-5 antibody [EPR14725] (ab184177)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (20)
ab184177 は 20 報の論文で使用されています。
- Henneberg LT et al. Activity-based profiling of cullin-RING E3 networks by conformation-specific probes. Nat Chem Biol 19:1513-1523 (2023). PubMed: 37653169
- Wang L et al. Culin5 aggravates hypoxic pulmonary hypertension by activating TRAF6/NF-κB/HIF-1α/VEGF. iScience 26:108199 (2023). PubMed: 37965157
- Li K et al. SOCS2 regulation of growth hormone signaling requires a canonical interaction with phosphotyrosine. Biosci Rep 42:N/A (2022). PubMed: 36398696
- Zeng J et al. A Hypoxia-Induced SCFFBXL1 E3 Ligase Ubiquitinates and Degrades the MEN1 Tumor Suppressor to Promote Colorectal Cancer Tumorigenesis. Cancer Res Treat 54:525-540 (2022). PubMed: 34237211
- Yin L et al. Early growth response-1 is a new substrate of the GSK3β-FBXW7 axis. Neoplasia 34:100839 (2022). PubMed: 36240645