Anti-Cullin 3/CUL-3 抗体 [EPR3196Y] (ab75851)
![Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.co.jp/ps/products/75/ab75851/Images/ab75851-2-ab75851FC.jpg "Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cullin 3/CUL-3 antibody [EPR3196Y]
Cullin 3/CUL-3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3196Y] to Cullin 3/CUL-3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, SH-SY5Y, PC12 and 3T3 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3196Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Cullin 3/CUL-3 antibody [EPR3196Y] - BSA and Azide free (ab239868)
- Alexa Fluor® 488 Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab320316)
- Alexa Fluor® 647 Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab320317)
- PE Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab320318)
- APC Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab320319)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75851の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/30 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/20000 - 1/100000. Predicted molecular weight: 89 kDa.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/30 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/20000 - 1/100000. Predicted molecular weight: 89 kDa. |
ターゲット情報
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機能
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. -
組織特異性
Widely expressed. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the cullin family. -
翻訳後修飾
Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
細胞内局在
Nucleus. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 8452 Human
- Entrez Gene: 26554 Mouse
- Entrez Gene: 301555 Rat
- Omim: 603136 Human
- SwissProt: Q13618 Human
- SwissProt: Q9JLV5 Mouse
- SwissProt: B5DF89 Rat
- Unigene: 372286 Human
see all -
別名
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
Overlay histogram showing HeLa cells stained with ab75851 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75851, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.co.jp/ps/products/75/ab75851/Images/ab75851wb.jpg)
Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)All lanes : Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Observed band size: 82,90 kDa why is the actual band size different from the predicted? -
![Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)](https://www.abcam.co.jp/ps/products/75/ab75851/Images/ab75851-3-benefits-of-recombinant-antibodies.png)
Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (ab75851)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab75851 は 16 報の論文で使用されています。
- Zhang F et al. Deacetylation of MTHFD2 by SIRT4 senses stress signal to inhibit cancer cell growth by remodeling folate metabolism. J Mol Cell Biol 14:N/A (2022). PubMed: 35349697
- Chen X et al. Long non-coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3. Int J Mol Med 48:N/A (2021). PubMed: 34036379
- Chen W et al. Cullin3 aggravates the inflammatory response of periodontal ligament stem cells via regulation of SHH signaling and Nrf2. Bioengineered 12:3089-3100 (2021). PubMed: 34193016
- Boro M et al. Thrombin-Par1 signaling axis disrupts COP9 signalosome subunit 3-mediated ABCA1 stabilization in inducing foam cell formation and atherogenesis. Cell Death Differ 28:780-798 (2021). PubMed: 32968199
- Xiu H et al. Neddylation Alleviates Methicillin-Resistant Staphylococcus aureus Infection by Inducing Macrophage Reactive Oxygen Species Production. J Immunol 207:296-307 (2021). PubMed: 34183370