Anti-Cullin 1/CUL-1 抗体 [EPR3102Y] (ab75812)
![Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)](https://www.abcam.co.jp/ps/products/75/ab75812/Images/ab75812-273679-anti-cullin-1-cul-1-antibody-epr3102y-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3102Y] to Cullin 1/CUL-1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cullin 1/CUL-1 antibody [EPR3102Y]
Cullin 1/CUL-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3102Y] to Cullin 1/CUL-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Cullin 1/CUL-1 aa 200-300. The exact sequence is proprietary.
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ポジティブ･コントロール
- 293T, A549 and T47D cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3102Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75812の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 90 kDa.
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| ICC/IF | (1) |
1/100 - 1/250.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 90 kDa. |
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ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for IHC-P or IP.
ターゲット情報
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機能
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
組織特異性
Expressed in lung fibroblasts. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the cullin family. -
翻訳後修飾
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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参照データベース
- Entrez Gene: 8454 Human
- Entrez Gene: 26965 Mouse
- Entrez Gene: 362356 Rat
- Omim: 603134 Human
- SwissProt: Q13616 Human
- SwissProt: Q9WTX6 Mouse
- Unigene: 146806 Human
- Unigene: 87611 Mouse
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別名
- CUL 1 antibody
- CUL-1 antibody
- CUL1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cullin 1/CUL-1 with ab75812 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab75812 at 1/400 was carried in PBS buffer for 1 hour at 22°C. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.
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![Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)](https://www.abcam.co.jp/ps/products/75/ab75812/Images/ab75812-90904-anti-cullin-1-cul-1-antibody-epr3102y-western-blot.gif)
Western blot - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)All lanes : Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812) at 1/2000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A549 cell lysate
Lane 3 : T47D cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)](https://www.abcam.co.jp/ps/products/75/ab75812/Images/ab75812-90905-anti-cullin-1-cul-1-antibody-epr3102y-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)ab75812 at 1/100 dilution staining Cullin 1/CUL-1 in HeLa cells by Immunofluorescence.
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![Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)](https://www.abcam.co.jp/ps/products/75/ab75812/Images/ab75812-4-benefits-of-recombinant-antibodies.png)
Anti-Cullin 1/CUL-1 antibody [EPR3102Y] (ab75812)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab75812 は 6 報の論文で使用されています。
- Zhao H et al. Targeted Inhibition of the E3 Ligase SCFSkp2/Cks1 Has Antitumor Activity in RB1-Deficient Human and Mouse Small-Cell Lung Cancer. Cancer Res 80:2355-2367 (2020). PubMed: 32265224
- Chung D et al. Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell Cycle 18:1135-1153 (2019). PubMed: 31057046
- Karpiyevich M et al. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites. PLoS Pathog 15:e1008086 (2019). PubMed: 31658303
- Bruckert WM et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). PubMed: 24126522
- Kainulainen M et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . PubMed: 24403578
- Chen H et al. DNA damage regulates UHRF1 stability via the SCF(ß-TrCP) E3 ligase. Mol Cell Biol 33:1139-48 (2013). PubMed: 23297342