Anti-CUG-BP1 抗体 [3B1] (ab9549)
![Immunocytochemistry - Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.co.jp/ps/products/9/ab9549/Images/ab9549-6-anti-cug-bp1-3b1-antibody-immunocytochemistry-hela-human.jpg "Immunocytochemistry - Anti-CUG-BP1 antibody [3B1] (ab9549)")
Key features and details
- Mouse monoclonal [3B1] to CUG-BP1
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CUG-BP1 antibody [3B1]
CUG-BP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3B1] to CUG-BP1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Pig
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免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa, HEK-293T and SHSY-5Y cell lysates; Human kidney and brain tissue lysates. ICC: HeLa cells. IHC: Human Breast cancer tissue
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3B1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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| ICC |
Use a concentration of 1 - 5 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
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ICC
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the CELF/BRUNOL family.
Contains 3 RRM (RNA recognition motif) domains. -
翻訳後修飾
Phosphorylated. Its phosphorylation status increases in senescent cells. -
細胞内局在
Nucleus. Cytoplasm. RNA-binding activity is detected in both nuclear and cytoplasmic compartments. - Information by UniProt
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参照データベース
- Entrez Gene: 10658 Human
- Entrez Gene: 13046 Mouse
- Entrez Gene: 362160 Rat
- Omim: 601074 Human
- SwissProt: Q92879 Human
- SwissProt: P28659 Mouse
- SwissProt: Q4QQT3 Rat
- Unigene: 595333 Human
see all -
別名
- 50 kDa Nuclear polyadenylated RNA binding protein antibody
- 50 kDa nuclear polyadenylated RNA-binding protein antibody
- Bruno like 2 antibody
see all
画像
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Immunocytochemistry - Anti-CUG-BP1 antibody [3B1] (ab9549)
ab9549 staining CUG-BP1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab9549 at 1 µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.co.jp/ps/products/9/ab9549/Images/ab9549-263271-anti-cug-bp1-antibody-3b1-western-blot.jpg)
Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)](https://www.abcam.co.jp/ps/products/9/ab9549/Images/ab9549-263635-anti-cug-bp1-antibody-3b1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)IHC image of CUG-BP1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9549, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab9549 は 21 報の論文で使用されています。
- Kang M et al. CYFIP2 p.Arg87Cys Causes Neurological Defects and Degradation of CYFIP2. Ann Neurol 93:155-163 (2023). PubMed: 36251395
- García-Puga M et al. Senescence plays a role in myotonic dystrophy type 1. JCI Insight 7:N/A (2022). PubMed: 36040809
- Hoefig KP et al. Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. Nat Commun 12:5208 (2021). PubMed: 34471108
- Suzuki T et al. Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development. Int J Mol Sci 21:N/A (2020). PubMed: 33297405
- Wu P et al. Tumor Cell-Derived TGFß1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA. Cancer Immunol Res 8:1470-1484 (2020). PubMed: 32999004